Identification of bovine viral diarrhea virus type 2 in cattle bull semen from southern India and its genetic characterization
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Title |
Identification of bovine viral diarrhea virus type 2 in cattle bull semen from southern India and its genetic characterization
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Creator |
Mishra, N.
Kalaiyarasu, S. Mallinath, K. C. Rajukumar, K. Rohit, K. Gautam, S. Venkatesha, M. D. Byregowda, S. M. |
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Subject |
Bovine viral diarrhoea, bull semen, genetic characterization, mandatory testing.
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Description |
Not Available
Although bovine viral diarrhoea virus (BVDV) is pre-valent in Indian cattle causing economic losses in cattle farming, its detection in bull semen has not yet been reported. Following passage of raw bull semen (n=4) on MDBK cells, testing for BVDV was done by antigen ELISA and real-time RT-PCR. BVDV type-2 (BVDV-2) was identified in three samples from south-ern India by real-time RT-PCR. Genetic typing of the 5-UTR sequences classified all the three BVDV strains as BVDV-2a subtype. These were found ge-netically closely related to the strains from USA, but divergent from the BVDV-2a strains from northern India. Phylogenetic analysis of Npro sequences con-firmed the findings. The results provide evidence of circulation of BVDV-2a strains in southern India. The detection of BVDV in bull semen from India high-lights the importance of mandatory testing of breeding bulls and bull semen for BVDV to minimize the risk of BVDV transmission. Not Available |
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Date |
2018-10-31T06:00:57Z
2018-10-31T06:00:57Z 2018-02-10 |
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Type |
Journal
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Identifier |
Mishra, N., Kalaiyarasu, S., Mallinath, K. C., Rajukumar, K., Rohit, K., Gautam, S., Venkatesha, M. D., Byregowda, S. M. (2018) Identification of bovine viral diarrhea virus type 2 in cattle bull semen from southern India and its genetic characterization. Current Science, 114 (3): 666-670.
0011-3891 http://krishi.icar.gov.in/jspui/handle/123456789/8804 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Current Science Association along with the Indian Academy of Sciences
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