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Identification of bovine viral diarrhea virus type 2 in cattle bull semen from southern India and its genetic characterization

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Title Identification of bovine viral diarrhea virus type 2 in cattle bull semen from southern India and its genetic characterization
Not Available
 
Creator Mishra, N.
Kalaiyarasu, S.
Mallinath, K. C.
Rajukumar, K.
Rohit, K.
Gautam, S.
Venkatesha, M. D.
Byregowda, S. M.
 
Subject Bovine viral diarrhoea, bull semen, genetic characterization, mandatory testing.
 
Description Not Available
Although bovine viral diarrhoea virus (BVDV) is pre-valent in Indian cattle causing economic losses in cattle farming, its detection in bull semen has not yet been reported. Following passage of raw bull semen (n=4) on MDBK cells, testing for BVDV was done by antigen ELISA and real-time RT-PCR. BVDV type-2 (BVDV-2) was identified in three samples from south-ern India by real-time RT-PCR. Genetic typing of the 5-UTR sequences classified all the three BVDV strains as BVDV-2a subtype. These were found ge-netically closely related to the strains from USA, but divergent from the BVDV-2a strains from northern India. Phylogenetic analysis of Npro sequences con-firmed the findings. The results provide evidence of circulation of BVDV-2a strains in southern India. The detection of BVDV in bull semen from India high-lights the importance of mandatory testing of breeding bulls and bull semen for BVDV to minimize the risk of BVDV transmission.
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Date 2018-10-31T06:00:57Z
2018-10-31T06:00:57Z
2018-02-10
 
Type Journal
 
Identifier Mishra, N., Kalaiyarasu, S., Mallinath, K. C., Rajukumar, K., Rohit, K., Gautam, S., Venkatesha, M. D., Byregowda, S. M. (2018) Identification of bovine viral diarrhea virus type 2 in cattle bull semen from southern India and its genetic characterization. Current Science, 114 (3): 666-670.
‎0011-3891
http://krishi.icar.gov.in/jspui/handle/123456789/8804
 
Language English
 
Relation Not Available;
 
Publisher Current Science Association along with the Indian Academy of Sciences