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Sero-diagnosis of surra exploiting recombinant VSG antigen based ELISA for surveillance

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Title Sero-diagnosis of surra exploiting recombinant VSG antigen based ELISA for surveillance
Not Available
 
Creator Sengupta PP
Rudramurthy GR
Ligi M
Roy M
Balamurugan V
Krishnamoorthy P
Nagalingam M
Singh L
Rahman H
 
Subject ELISA
Surra
Trypanosoma evansi
Variable surface glycoprotein
 
Description Not Available
Trypanosoma evansi, a haemoflagellate, causes "surra" an important chronic wasting disease of a wide range of wild and domestic herbivorous and carnivorous animals including cattle, buffaloes, camels, horses, etc. The untreated recovered animal can act as a carrier without exhibiting the disease symptoms and can be a source of infection to healthy animals. The diagnosis and subsequent treatment of the carrier animals is helpful to curb the disease. As the parasitaemia in carrier animals is very scanty, the conventional blood smear examination, which is widely practiced in the field, cannot detect such condition. For this purpose improved diagnostics are very much useful for mass sero-screening test such as ELISA. In the present study, the VSG of T. evansi was expressed in prokaryotic system (E. coli) and thereafter its immunoreactivity has been evaluated in immuno blot and enzyme immuno assay. The expressed protein showed 95.6% sensitivity, 98.0% specificity and 0.93 Cohen's kappa value, when compared with standard antigens. The developed antigen has also been validated with field serum samples from bovine, camel and horse collected from different states of India. The data showed that the developed recombinant antigen can be a diagnostic tool to detect carrier animals as well as control of the disease.
Not Available
 
Date 2018-11-01T08:22:14Z
2018-11-01T08:22:14Z
2014-10-15
 
Type Research Paper
 
Identifier Not Available
0304-4017
http://krishi.icar.gov.in/jspui/handle/123456789/8994
 
Language English
 
Relation Not Available;
 
Publisher Elsevier Publications