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Flagellar antigen based CI-ELISA for sero-surveillance of surra

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Title Flagellar antigen based CI-ELISA for sero-surveillance of surra
Not Available
 
Creator Ligi M
Sengupta PP
Rudramurthy GR
Rahman H
 
Subject CI-ELISA
Flagellar antigen
Monoclonal antibody
ROC analysis
Surra
 
Description Not Available
Trypanosoma evansi causes a disease known as 'surra' in wide range of domesticated and wild animals including cattle, buffaloes, horses, camels etc. The disease is transmitted through the bites of haematophagous tabanid flies and is characterized by undulating fever, chronic progressive weakness, and hypoglycemia leading to low productivity in animals. In the present study, monoclonal antibodies (MAbs) have been produced (IgG3 sub-type) against purified flagellar (FLA) protein of T. evansi and its immunoreactivity was evaluated by serological tests. MAb and purified protein were then exploited in the development of CI-ELISA and the diagnostic potentiality of the new ELISA test has been evaluated using 1230 sera samples from field animals including cattle, buffaloes, camels, horses and donkeys. The statistical analysis of the data showed optimum combination of sensitivity and specificity at 95.8 and 94.4, respectively. The positive-negative cut off percentage inhibition (PI) value was found to be >55, with a Cohen's Kappa value of 0.83. The study showed that the new assay has potential for application in sero-diagnosis as well as sero-surveillance of surra.
Not Available
 
Date 2018-11-05T03:31:34Z
2018-11-05T03:31:34Z
2016-01-28
 
Type Research Paper
 
Identifier Not Available
0304-4017
http://krishi.icar.gov.in/jspui/handle/123456789/9266
 
Language English
 
Relation Not Available;
 
Publisher Elsevier