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Full-Genome sequencing as a basis for molecular epidemiology studies of bluetongue virus in India.

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Title Full-Genome sequencing as a basis for molecular epidemiology studies of bluetongue virus in India.
Not Available
 
Creator Maan S
Maan NS
Belaganahalli MN
Rao PP
Singh KP
Hemadri D
Putty K
Kumar A
Batra K
Krishnajyothi Y
Chandel BS
Reddy GH
Nomikou K
Reddy YN
Attoui H
Hegde NR
Mertens PP
 
Subject Full-Genome Sequencing
Molecular Epidemiology
Studies
Bluetongue Virus
India
 
Description Not Available
Since 1998 there have been significant changes in the global distribution of bluetongue virus (BTV). Ten previously exotic BTV serotypes have been detected in Europe, causing severe disease outbreaks in naïve ruminant populations. Previously exotic BTV serotypes were also identified in the USA, Israel, Australia and India. BTV is transmitted by biting midges (Culicoides spp.) and changes in the distribution of vector species, climate change, increased international travel and trade are thought to have contributed to these events. Thirteen BTV serotypes have been isolated in India since first reports of the disease in the country during 1964. Efficient methods for preparation of viral dsRNA and cDNA synthesis, have facilitated full-genome sequencing of BTV strains from the region. These studies introduce a new approach for BTV characterization, based on full-genome sequencing and phylogenetic analyses, facilitating the identification of BTV serotype, topotype and reassortant strains. Phylogenetic analyses show that most of the equivalent genome-segments of Indian BTV strains are closely related, clustering within a major eastern BTV 'topotype'. However, genome-segment 5 (Seg-5) encoding NS1, from multiple post 1982 Indian isolates, originated from a western BTV topotype. All ten genome-segments of BTV-2 isolates (IND2003/01, IND2003/02 and IND2003/03) are closely related (>99% identity) to a South African BTV-2 vaccine-strain (western topotype). Similarly BTV-10 isolates (IND2003/06; IND2005/04) show >99% identity in all genome segments, to the prototype BTV-10 (CA-8) strain from the USA. These data suggest repeated introductions of western BTV field and/or vaccine-strains into India, potentially linked to animal or vector-insect movements, or unauthorised use of 'live' South African or American BTV-vaccines in the country. The data presented will help improve nucleic acid based diagnostics for Indian serotypes/topotypes, as part of control strategies.
Not Available
 
Date 2018-11-05T03:40:35Z
2018-11-05T03:40:35Z
2015-06-29
 
Type Research Paper
 
Identifier Not Available
1932-6203
http://krishi.icar.gov.in/jspui/handle/123456789/9270
 
Language English
 
Relation Not Available;
 
Publisher Public Library of Science