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Duplex PCR for specific detection of Escherichia coli and its differentiation from other Enterobacteriaceae.

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Title Duplex PCR for specific detection of Escherichia coli and its differentiation from other Enterobacteriaceae.
Not Available
 
Creator Mitra SD
Wilfred AD
Tewari R
Nimita VC
Bhuvana M
Krithiga N
Shome BR
Rahman H
 
Subject Duplex polymerase chain reaction
Enterobacteriaceae
Escherichia coli
 
Description Not Available
Escherichia coli is a normal inhabitant of gastrointestinal tract of humans and animals and one of the mostimportant causes of bovine mastitis. Definitive identification of E. coli from other members of Enterobacteriaceaeremains ambiguous. The present study describes a duplex PCR, targeting 2 housekeeping genes, the lacy (lactosepermease) and phoA (alkaline phosphatase) for the reliable detection of E. coli that enables its differentiation frombiochemically and phylogenetically related bacteria. The assay was evaluated employing 19 ATCC (American typeculture collection) reference strains of Enterobacteriaceae family. Validation of the assay with E. coli (154) isolatedfrom milk and faeces rendered the assay to be specific. The results suggest that the technique can be used foraccurate detection of E. coli and thus can be adapted for testing bacteriological safety of milk, for field applications,and in laboratories handling clinical samples. This PCR (polymerase chain reaction) can successfully distinguishE. coli including E. coli O157 from Shigella spp and other related enterobacteria, emphasizing its relevance andutility in studies related to E. coli infection
Not Available
 
Date 2018-11-05T03:46:32Z
2018-11-05T03:46:32Z
2015-08-01
 
Type Research Paper
 
Identifier Not Available
0367-8318
http://krishi.icar.gov.in/jspui/handle/123456789/9272
 
Language English
 
Relation Not Available;
 
Publisher Indian Council of Agricultural Research