Duplex PCR for specific detection of Escherichia coli and its differentiation from other Enterobacteriaceae.
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Title |
Duplex PCR for specific detection of Escherichia coli and its differentiation from other Enterobacteriaceae.
Not Available |
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Creator |
Mitra SD
Wilfred AD Tewari R Nimita VC Bhuvana M Krithiga N Shome BR Rahman H |
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Subject |
Duplex polymerase chain reaction
Enterobacteriaceae Escherichia coli |
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Description |
Not Available
Escherichia coli is a normal inhabitant of gastrointestinal tract of humans and animals and one of the mostimportant causes of bovine mastitis. Definitive identification of E. coli from other members of Enterobacteriaceaeremains ambiguous. The present study describes a duplex PCR, targeting 2 housekeeping genes, the lacy (lactosepermease) and phoA (alkaline phosphatase) for the reliable detection of E. coli that enables its differentiation frombiochemically and phylogenetically related bacteria. The assay was evaluated employing 19 ATCC (American typeculture collection) reference strains of Enterobacteriaceae family. Validation of the assay with E. coli (154) isolatedfrom milk and faeces rendered the assay to be specific. The results suggest that the technique can be used foraccurate detection of E. coli and thus can be adapted for testing bacteriological safety of milk, for field applications,and in laboratories handling clinical samples. This PCR (polymerase chain reaction) can successfully distinguishE. coli including E. coli O157 from Shigella spp and other related enterobacteria, emphasizing its relevance andutility in studies related to E. coli infection Not Available |
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Date |
2018-11-05T03:46:32Z
2018-11-05T03:46:32Z 2015-08-01 |
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Type |
Research Paper
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Identifier |
Not Available
0367-8318 http://krishi.icar.gov.in/jspui/handle/123456789/9272 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Indian Council of Agricultural Research
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