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Analysis of the crow lung transcriptome in response to infection with highly pathogenic H5N1 avian influenza virus

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Title Analysis of the crow lung transcriptome in response to infection with highly pathogenic H5N1 avian influenza virus
Not Available
 
Creator Vijaykumar P
Mishra A
Ranaware PB
Kolte AP
Kulkarni DD
Burt DW
Raut AA
 
Subject Avian influenza virus infection
De novo transcriptomics
High-throughput RNA sequencing
Host immune response
Jungle crows
 
Description Not Available
The highly pathogenic avian influenza (HPAI) H5N1 virus, currently circulating in Asia, causes severe disease in domestic poultry as well as wild birds like crow. However, the molecular pathogenesis of HPAIV infection in crows and other wild birds is not well known. Thus, as a step to explore it, a comprehensive global gene expression analysis was performed on crow lungs, infected with HPAI H5N1 crow isolate (A/Crow/India/11TI11/2011) using high throughput next generation sequencing (NGS) (GS FLX Titanium XLR70). The reference genome of crow is not available, so RNA seq analysis was performed on the basis of a de novo assembled transcriptome. The RNA seq result shows, 4052 genes were expressed uniquely in noninfected, 6277 genes were expressed uniquely in HPAIV infected sample and of the 6814 genes expressed in both samples, 2279 genes were significantly differentially expressed. Our transcriptome profile data allows for the ability to understand the molecular mechanism behind the recent lethal HPAIV outbreak in crows which was, until recently, thought to cause lethal infections only in gallinaceous birds such as chickens, but not in wild birds. The pattern of differentially expressed genes suggest that this isolate of H5N1 virus evades the host innate immune response by attenuating interferon (IFN)-inducible signalling possibly by down regulating the signalling from type I IFN (IFNAR1 and IFNAR2) and type II IFN receptors, upregulation of the signalling inhibitors suppressor of cytokine signalling 1 (SOCS1) and SOCS3 and altering the expression of toll-like receptors (TLRs). This may be the reason for disease and mortality in crows
Not Available
 
Date 2018-11-22T11:38:10Z
2018-11-22T11:38:10Z
2015-01-01
 
Type Research Paper
 
Identifier Vijaykumar P, Mishra A, Ranaware PB, Kolte AP, Kulkarni DD, Burt DW and Raut AA. 2015. Analysis of the crow lung transcriptome in response to infection with highly pathogenic H5N1 avian influenza virus. Gene, 559:77-85.
0378-1119
http://krishi.icar.gov.in/jspui/handle/123456789/12851
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER