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Role of calcium and magnesium administration on sex ratio skewing, follicular fluid protein profiles and steroid hormone level and oocyte transcripts expression pattern in Wistar rat

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Title Role of calcium and magnesium administration on sex ratio skewing, follicular fluid protein profiles and steroid hormone level and oocyte transcripts expression pattern in Wistar rat
Not Available
 
Creator Arangasamy A
Selvarju S
Parthipan S
Somashekar L
Rajendran D
Ravindra JP
 
Subject Calcium
Magnesium
Reproduction
Sex ratio
Testosterone
Wistar rat
 
Description Not Available
An attempt was made with 2% calcium and 0.4% magnesium administration to assess the changes in sex ratio
skewing in Wistar rat. The results indicated that there was a significant change in sex ratio skewing towards females (11.7% and sex ratio skewed was 1:1.3). There was a significant reduction in serum testosterone levels (P< 0.01)between control (0.35±0.04 ng/ml) and treatment (0.20±0.08 ng/ml) groups and a nonsignificant decrease in follicular fluid. Significant changes were observed in serum T4 level between control (52.32±2.74 nmol/L) and treatment (33.18±3.75 nmol/L) groups. Variation in follicular fluid protein profiles were observed by 2D gel electrophoresis. Differential expression of protein with high molecular weight (52 and 80 kDa) and low pH is not present in treatment group and protein with low molecular weight (10 and 11 kDa) and high pH were observed in treatment group. The expression of transcripts for BMP-15 was 11.5 folds higher and GDF-9 was 3.5 folds higher in treatment group than control. Our results suggested that possible mechanism to favour female offspring could be reduction in serum testosterone and lowering follicular fluid protein molecular weight along with high pH (haemoglobin subunit beta1protein (Mr) 15.9 kDa; cytochrome b-c1 complex subunit 8 (Mr) 9.8 kDa; asparagine synthetase (Mr) 64.7 kDa;metal transporter CNNM4(Mr) 87.4 kDa) and increased mRNA expression of BMP-15 and GDF-9 in oocyte.
ICAR-National Institute of Animal Nutrition and Physiology
 
Date 2018-11-22T23:03:55Z
2018-11-22T23:03:55Z
2015-01-01
 
Type Research Paper
 
Identifier Arangasamy A, Selvarju S, Parthipan S, Somashekar L, Rajendran D and Ravindra JP. 2015. Role of calcium and magnesium administration on sex ratio skewing, follicular fluid protein profiles and steroid hormone level and oocyte transcripts expression pattern in Wistar rat. Indian Journal of Animal Sciences, 85:247-249.
0367-8318
http://krishi.icar.gov.in/jspui/handle/123456789/12860
 
Language English
 
Relation Not Available;
 
Publisher Not Available