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Effect of nicotinic acetylcholine receptor alpha 1 (nAChRα1) peptides on rabies virus infection in neuronal cells

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Title Effect of nicotinic acetylcholine receptor alpha 1 (nAChRα1) peptides on rabies virus infection in neuronal cells
Not Available
 
Creator Sajjanar B
Saxena S
Bisht D
Singh AK
Manjunathareddy GB
Singh R
Singh RP
Kumar S
 
Subject Neuroblastoma cells
Peptides
Rabies virus
Receptor subunits
 
Description Not Available
Rabies virus (RABV) is neurotropic and causes acute progressive encephalitis. Herein, we report the interaction of nAChRα1-subunit peptides with RABV and the effect of these peptides on RABV infection in cultured neuronal cells. Peptide sequences derived from torpedo, bovine, human and rats were synthesized and studied for their interactions with RABV using virus capture ELISA and peptide immunofluorescence. The results showed specific binding of the nAChRα1-subunit peptides to the RABV. In the virus adsorption assay, these peptides were found to inhibit the attachment of the RABV to the neuronal cells. The nAChRα1-subunit peptides inhibited the RABV infection and reduced viral gene expression in the cultured neuroblastoma (N2A) cells. Torpedo peptide sequence (T-32) had highest antiviral effect (IC50=14±3.01μM) compared to the other peptides studied. The results of the study indicated that nAChRα1-subunit peptides may act as receptor decoy molecules and inhibit the binding of virus to the native host cell receptors and hence may reduce viral infection.
Not Available
 
Date 2018-11-05T04:04:19Z
2018-11-05T04:04:19Z
2016-06-01
 
Type Research Paper
 
Identifier Not Available
0143-4179
http://krishi.icar.gov.in/jspui/handle/123456789/9281
 
Language English
 
Relation Not Available;
 
Publisher Elsevier