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Expression of glycoprotein gene of rabies virus and evaluation of recombinant protein for seromonitoring of vaccinal antibodies in dogs

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Title Expression of glycoprotein gene of rabies virus and evaluation of recombinant protein for seromonitoring of vaccinal antibodies in dogs
Not Available
 
Creator Sharada R
Isloor SI
Balamurugan V
Veeresh B
Suryanarayana V
Manisha R
Rathnamma D
Satyanarayana M
 
Subject Expression
Glycoprotein gene
Rabies virus
Evaluation
Recombinant protein
Seromonitoring
Vaccinal antibodies
Dogs
 
Description Not Available
Rickettsia felis bacterium has been observed since the
early twentieth century in the cat flea. It was grown for the first
time in my laboratory on cell Xenopus then insect cells particularly
Aedes albopictus cells. It grows only at temperature under 30 ◦C.
First described in California, the bacterium is present in the whole
world. For a long time it was considered a rare disease associated
with fleas. In fact, in recent years, Rickettsia felis was found as the
most frequently identified bacteria in patients with fever in tropical
countries after malaria. These data were confirmed in West
Africa and East Africa by different teams. Rickettsia felis has also
been detected in tropical and tropical areas of South America, Asia
and Oceania. Rickettsia felis and is the most common rickettsial currently
in the world and very frequently associated with fever in
the tropics and cough but no rash. In contrast controls without
fever, can cause at a lower but significant level, the presence of
Rickettsia felis DNA. So these circulations of Rickettsia felis in the
blood that are not always associated with fever. Possible vectors in
tropical areas may be mosquitoes in Africa. Rickettsia felis has the
same epidemiological distribution as malaria. Rickettsia felis was
found in Anopheles species and Aedes albopictus. A mouse experimental
model was established showing that Rickettsia felis could be
vectorized by Anopheles gambiae. Rickettsia felis, finally, recently,
was found in book lice in the dust, which further complicates the
epidemiological cycle of this bacterium. R. felis may be the most
common bacteria emerging virus (RABV) dependent and laborious Rapid Fluorescent Focus
Inhibition Test (RFFIT) is employed in selected laboratories in India
to monitor the vaccinal antibodies. This situation demands an alternative
rapid, sensitive, specific and user friendly test. Hence, this
study was undertaken to express the recombinant glycoprotein
(rRVL-G) of Dr. Larghi’s strain of RABV and evaluate it’s diagnostic
potential.
Not Available
 
Date 2018-11-05T04:06:49Z
2018-11-05T04:06:49Z
2016-03-29
 
Type Research Paper
 
Identifier Not Available
1201-9712
http://krishi.icar.gov.in/jspui/handle/123456789/9282
 
Language English
 
Relation Not Available;
 
Publisher Elsevier