Effect of stearic acid on in vitro development of sheep oocytes
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Title |
Effect of stearic acid on in vitro development of sheep oocytes
Not Available |
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Creator |
Farman M
Nandi S Kumar GV Tripathi SK Gupta PSP |
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Subject |
IVF
Metabolic stressors NEFA Stearic acid |
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Description |
Not Available
The present study was undertaken to study the effect of stearic acid (SA), a non esterified fatty acid (NEFA) on oocyte development of ewes (Ovis aries). Sheep oocytes were matured in vitro in the presence of different concentration of stearic acid (0 µM, 10 µM, 20 µM, and 30 µM) for 24h. After in vitro maturation, oocytes were in vitro inseminated with cauda epidydimus sperm processed with BO medium with concentration of 2 million sperms/ml and cultured on oviductal cell culture for 8 days. Oocytes were evaluated for cleavage and fertilization rates and after 7–8 days post insemination zygotes were evaluated for moruale /blastocyst stages. The maturation, cleavage and morulae/ blastocyst production rates were significantly lowered in media containing 20 µM stearic acid. Increment of stearic acid to 30 µM in media further reduced the maturation, cleavage and morulae/ blastocyst production. In conclusion, the metabolic stressors NEFA (SA) impaired the maturation, viability, cleavage and embryo production rates at the level 20 µM in ewe DBT, GOI |
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Date |
2018-11-22T23:39:27Z
2018-11-22T23:39:27Z 2015-06-01 |
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Type |
Research Paper
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Identifier |
Farman M, Nandi S, Kumar GV, Tripathi SK and Gupta PSP. 2015. Effect of stearic acid on in vitro development of sheep oocytes. Indian Journal of Animal Sciences, 85:584-587.
0367-8318 http://krishi.icar.gov.in/jspui/handle/123456789/12868 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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