Record Details

Effect of stearic acid on in vitro development of sheep oocytes

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Effect of stearic acid on in vitro development of sheep oocytes
Not Available
 
Creator Farman M
Nandi S
Kumar GV
Tripathi SK
Gupta PSP
 
Subject IVF
Metabolic stressors
NEFA
Stearic acid
 
Description Not Available
The present study was undertaken to study the effect of stearic acid (SA), a non esterified fatty acid (NEFA) on oocyte development of ewes (Ovis aries). Sheep oocytes were matured in vitro in the presence of different concentration of stearic acid (0 µM, 10 µM, 20 µM, and 30 µM) for 24h. After in vitro maturation, oocytes were in vitro inseminated with cauda epidydimus sperm processed with BO medium with concentration of 2 million sperms/ml and cultured on oviductal cell culture for 8 days. Oocytes were evaluated for cleavage and fertilization rates and after 7–8 days post insemination zygotes were evaluated for moruale /blastocyst stages. The maturation, cleavage and morulae/ blastocyst production rates were significantly lowered in media containing 20 µM stearic acid. Increment of stearic acid to 30 µM in media further reduced the maturation, cleavage and morulae/ blastocyst production. In conclusion, the metabolic stressors NEFA (SA) impaired the maturation, viability, cleavage and embryo production rates at the level 20 µM in ewe
DBT, GOI
 
Date 2018-11-22T23:39:27Z
2018-11-22T23:39:27Z
2015-06-01
 
Type Research Paper
 
Identifier Farman M, Nandi S, Kumar GV, Tripathi SK and Gupta PSP. 2015. Effect of stearic acid on in vitro development of sheep oocytes. Indian Journal of Animal Sciences, 85:584-587.
0367-8318
http://krishi.icar.gov.in/jspui/handle/123456789/12868
 
Language English
 
Relation Not Available;
 
Publisher Not Available