Polyanhydride nanovaccine against swine influenza virus in pigs
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Title |
Polyanhydride nanovaccine against swine influenza virus in pigs
Not Available |
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Creator |
Dhakal S
Goodman J Bondra K Lakshmanappa YS Hiremath J Shyu DL Ouyang K Kang KI Krakowka S Wannemuehler MJ Won Lee C Narasimhan B Renukaradhya GJ |
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Subject |
Swine influenza virus
Polyanhydride nanoparticles Intranasal Immunity Pig |
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Description |
Not Available
We have recently demonstrated the effectiveness of an influenza A virus (IAV) subunit vaccine based onbiodegradable polyanhydride nanoparticles delivery in mice. In the present study, we evaluated the effi-cacy of 200 nm polyanhydride nanoparticles encapsulating inactivated swine influenza A virus (SwIAV)as a vaccine to induce protective immunity against a heterologous IAV challenge in pigs. Nursery pigswere vaccinated intranasally twice with inactivated SwIAV H1N2 (KAg) or polyanhydride nanoparticle-encapsulated KAg (KAg nanovaccine), and efficacy was evaluated against a heterologous zoonotic virulentSwIAV H1N1 challenge. Pigs were monitored for fever daily. Local and systemic antibody responses,antigen-specific proliferation of peripheral blood mononuclear cells, gross and microscopic lung lesions,and virus load in the respiratory tract were compared among the groups of animals. Our pre-challengeresults indicated that KAg nanovaccine induced virus-specific lymphocyte proliferation and increasedthe frequency of CD4+CD8aa+T helper and CD8+cytotoxic T cells in peripheral blood mononuclear cells.KAg nanovaccine-immunized pigs were protected from fever following SwIAV challenge. In addition, pigsimmunized with the KAg nanovaccine presented with lower viral antigens in lung sections and had 6 to8-fold reduction in nasal shedding of SwIAV four days post-challenge compared to control animals.Immunologically, increased IFN-csecreting T lymphocyte populations against both the vaccine and chal-lenge viruses were detected in KAg nanovaccine-immunized pigs compared to the animals immunizedwith KAg alone. However, in the KAg nanovaccine-immunized pigs, hemagglutination inhibition, IgGand IgA antibody responses, and virus neutralization titers were comparable to that in the animals immu-nized with KAg alone. Overall, our data indicated that intranasal delivery of polyanhydride-based SwIAVnanovaccine augmented antigen-specific cellular immune response in pigs, with promise to induce cross-protective immunity Not Available |
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Date |
2018-11-05T09:24:21Z
2018-11-05T09:24:21Z 2017-02-22 |
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Type |
Research Paper
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Identifier |
Not Available
1550-6606 http://krishi.icar.gov.in/jspui/handle/123456789/9352 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Elsevier
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