Production, purification and characterization of novel laccase produced by Schizophyllum commune NI_07 with potential for delignification of crop residues
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
Production, purification and characterization of novel laccase produced by Schizophyllum commune NI_07 with potential for delignification of crop residues
Not Available |
|
Creator |
Kumar VP
Naik C Sridhar M |
|
Subject |
laccase
immobilization Schizophyllum commune delignification |
|
Description |
Not Available
The rumen microbial utilization of energy-rich cell walls of crop residues is hindered by the presence of lignin, which limitsits overall digestion process and significantly influences animal performance. In the frame of the development of a bioprocess using the competences of white rot fungi to enhance digestibility of crop residues by delignification, a new strain of Schizophyllum commune NI-07 was isolated. The sole lignolytic activity detected in submerged culture was laccase which increased 3-fold after immobilization of fungus on polyurethane foam cubes. The enzyme was purified 42-fold by employing ammonium sulphate precipitation and size exclusion chromatography on Sephadex G-50 to a specific activity of 15930 U/mg of protein and had a molecular mass of 75 kDa. The laccase obtained from submerged culture medium after cul- tivation of immobilized S. commune NI-07, exhibited considerably higher pH and thermostability and affinity for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) compared to those obtained without immobilization of the fungus. Highly significant (P < 0.05) improvement in the in vitro dry matter digestibility was obtained in 5 common crop residues treated with fungal laccases. We prove the potential of laccase obtained from S. commune NI-07 in enhancement of digestibility of crop residues by way of delignification. |
|
Date |
2018-11-22T23:55:12Z
2018-11-22T23:55:12Z 2015-01-01 |
|
Type |
Research Paper
|
|
Identifier |
Kumar VP, Naik C and Sridhar M. 2015. Production, purification and characterization of novel laccase produced by Schizophyllum commune NI_07 with potential for delignification of crop residues. Applied Biochemistry and Microbiology, 51:432-441.
0003-6838 http://krishi.icar.gov.in/jspui/handle/123456789/12875 |
|
Language |
English
|
|
Relation |
Not Available;
|
|
Publisher |
Springer
|
|