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Phylogenetic analysis of glycoprotein B gene sequences of bovine herpesvirus 1 isolates from India reveals the predominance of subtype 1.1.

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Title Phylogenetic analysis of glycoprotein B gene sequences of bovine herpesvirus 1 isolates from India reveals the predominance of subtype 1.1.
Not Available
 
Creator Patil SS
Prajapati A
Hemadri D
Suresh KP
Desai GS
Reddy GBM
Chandranaik BM
Ranganatha S
Rahman H
 
Subject bovine herpesvirus 1-1
glycoprotein B
India
infectious bovine rhinotracheitis
phylogenetic analysis
sequencing
subtype 1.1
 
Description Not Available
Aim:
This study was conducted for the isolation and molecular characterization of bovine herpesvirus 1 (BoHV-1) isolated from the nasal and vaginal swabs collected from naturally infected cattle showing clinical symptoms of the respiratory disease.

Materials and Methods:
Isolation of BoHV-1 virus performed on clinical samples collected from 65 cattle from five states of India. The BoHV-1 isolates were further confirmed by polymerase chain reaction (PCR) using primers specific for glycoprotein B (gB) genomic region. PCR amplification was performed using previously published gB gene-specific primer pairs. gB PCR amplicons obtained from all isolates were sequenced, and phylogenetic analysis was performed using software.

Results:
A total of 12 samples were found positive in cell culture isolation. 11 isolates showed the visible cytopathic effect on Madin-Darby bovine kidney after 72 h. Partial sequence analysis of gB gene of all isolates revealed 99.0-100% homology between them. All isolates showed 99.2-99.8% homology with Cooper stain.

Conclusion:
BoHV-1.1 is the predominant circulating subtype of BoHV in India, and all isolates have homology with Cooper stain.
Not Available
 
Date 2018-11-06T08:40:26Z
2018-11-06T08:40:26Z
2016-12-01
 
Type Research Paper
 
Identifier Not Available
2307-8316
http://krishi.icar.gov.in/jspui/handle/123456789/9566
 
Language English
 
Relation Not Available;
 
Publisher Veterinary World