An inhibition enzyme immuno assay exploring recombinant invariant surface glycoprotein and monoclonal antibodies for surveillance of surra in animals
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Title |
An inhibition enzyme immuno assay exploring recombinant invariant surface glycoprotein and monoclonal antibodies for surveillance of surra in animals
Not Available |
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Creator |
Rudramurthy GR
Sengupta PP Ligi M Rahman H |
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Subject |
ISG; Inhibition ELISA; Monoclonal antibody; Recombinant ISG; Serodiagnosis; Trypanosoma evansi
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Description |
Not Available
The present study is aimed at the development of inhibition ELISA (I-ELISA) exploring monoclonal antibodies (MAbs) and recombinant invariant surface glycoprotein. The extracellular domain (ED) of invariant surface glycoprotein (ISG-75) from Trypanosoma evasni has been heterologously expressed in Pichia pastoris (X-33). The recombinant ISG-75 (rISG-75ED) was characterized by immunoblot and ELISA, followed by the production of MAbs against rISG-75ED. The MAbs were characterized by immunoblot and then explored in the development of I-ELISA for the detection of surra. The diagnostic potential of the developed test has been evaluated using 1192 field sera sample including cattle, buffalo, donkey, horse and camel. The statistical analysis of the data showed optimum combination of diagnostic sensitivity and specificity at 98.8% and 99.2% respectively, with cut-off percentage inhibition (PI) value of >45. The Cohen's kappa coefficient of agreement was found to be 0.98. Hence, the diagnostic test developed in the present study can be exploited as a potential and reliable tool in the serodiagnosis and surveillance of surra in animals. Not Available |
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Date |
2018-11-06T08:36:23Z
2018-11-06T08:36:23Z 2017-03-01 |
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Type |
Research Paper
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Identifier |
Not Available
1432-0800 http://krishi.icar.gov.in/jspui/handle/123456789/9564 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Elsevier
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