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Standardization of an ELISA using recombinant nucleocapsid protein to quantify antibody response against Newcastle Disease Virus in chickens

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Title Standardization of an ELISA using recombinant nucleocapsid protein to quantify antibody response against Newcastle Disease Virus in chickens
Not Available
 
Creator Logeswari S
John Kirubaharan J
Rajkumar R
Rajendran D
 
Subject Newcastle disease
NP gene
Expressed protein
E.coli
ELISA
HI
 
Description Not Available
The coding sequence of NP gene was amplified by RT-PCR, cloned and expressed in prokaryotic expression system-E. coli. The crude extract of expressed protein was purified, extensively dialyzed and further purified by expanded bed adsorption chromatography. Three indirect ELISA using recombinant NP were standardized and the antibody titres against NDV in serum samples were found to correlate among these three ELISAs. The correlation coefficient values were 0.3 to 0.6 which were higher than table values for correlation coefficient at 1% level. The empirical ROC values were observed to be 0.929, 1.0 and 1.0 for comparisons between RP-ELISA versusHI, RP-ELISA versus WVP-ELISA and R P-ELISA versus PEPELISA respectively which are more than the standard value of 0.8.
Not Available
 
Date 2018-11-23T00:42:36Z
2018-11-23T00:42:36Z
2016-01-01
 
Type Research Paper
 
Identifier Logeswari S, John Kirubaharan J, Rajkumar R and Rajendran D. 2016. Standardization of an ELISA using recombinant nucleocapsid protein to quantify antibody response against Newcastle Disease Virus in chickens. Research Journal of Biotechnology, 11:75-80.
0973-6263
http://krishi.icar.gov.in/jspui/handle/123456789/12878
 
Language English
 
Relation Not Available;
 
Publisher Dr. Jyoti Garg