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Development of ELISA exploring recombinant variable surface glycoprotein for diagnosis of surra in animals

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Title Development of ELISA exploring recombinant variable surface glycoprotein for diagnosis of surra in animals
Not Available
 
Creator Sengupta PP
Ligi M
Rudramurthy GR
Balamurugan V
Rahman H
 
Subject Diagnosis
ELISA
rVSG
surra
Trypanosoma evansi
 
Description Not Available
In the present study, the variable surface glycoprotein
(VSG) gene of Trypanosoma evansi was cloned and
expressed in Pichia pastoris (X-33). The diagnostic
potential of recombinant VSG (rVSG) in ELISA has
been determined using 1818 field sera samples collected
from different species across different states of
India. The developed test was compared with the
standard reference test such as, CATT/T. evansi;
moreover, the new assay was also compared in ELISA
using VSG RoTat 1.2 antigen. The diagnostic sensitivity
and specificity of recombinant protein were found
to be 95.4% and 93.8% respectively, with Cohen’s
kappa value of 0.86. The epidemiological study revealed
varied prevalence of surra in different species
and across different geographical regions of India.
Cattle experienced higher prevalence of surra with
42.2% seropositivity from eastern region of India,
whereas camel showed 19.9% seropositivity from
Rajasthan. Hence, the present study is useful as
Not Available
 
Date 2018-11-06T08:35:41Z
2018-11-06T08:35:41Z
2016-08-04
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/9561
 
Language English
 
Relation Not Available;
 
Publisher RESEARCH COMMUNICATIONS