Effect of fibroblast growth factor 2 (FGF2) and insulin transferrin selenium (ITS) on in vitro maturation, fertilization and embryo development in sheep
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Title |
Effect of fibroblast growth factor 2 (FGF2) and insulin transferrin selenium (ITS) on in vitro maturation, fertilization and embryo development in sheep
Not Available |
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Creator |
Mondal S
Mor A Reddy IJ Nandi S Gupta PSP |
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Subject |
fibroblast growth factor 2
insulin transferrin selenium oocyte embryo sheep |
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Description |
Not Available
The present study evaluated the effect of fibroblast growth factor 2 (FGF2) and insulin-transferrin-selenium (ITS) to the in vitro maturation and embryo culture media on ovine oocyte maturation, cleavage and embryo development. Oocytes having more than five layers of unexpanded cumulus cells and granular homogenous ooplasm were cultured into 50 μL droplets of eight different culture systems: (i) TCM-199 (Tissue Culture Medium-199); (ii) TCM-199+10 ng/mL FGF2; (iii) TCM-199+20 ng/mL FGF2; (iv) TCM-199+30 ng/mL FGF2; (v) TCM-199+10 ng/mL ITS; (vi) TCM-199+20 ng/mL ITS; (vii) TCM-199+30 ng/mL ITS and (viii) TCM-199+20 ng/mL ITS+20 ng/mL FGF2 in a CO2 incubator at 38.50C for 24 h. All the oocyte culture media were supplemented with 10% FBS, FSH (10 μg/mL) and gentamicin (50 µg/mL). The maturation rate was assessed based on the degree of expansion of cumulus cells and identifying first polar body extrusion into perivitelline space. The matured oocytes were inseminated with 1 to 2 million spermatozoa/mL in Brackett and Oliphant medium and the cleavage rate was checked after 42-48 h post insemination and further cultured for 6-7 days. Maturation and cleavage rates were significantly higher (P National Agricultural Science Fund |
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Date |
2018-11-23T03:07:17Z
2018-11-23T03:07:17Z 2015-01-01 |
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Type |
Research Paper
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Identifier |
Mondal S, Mor A, Reddy IJ, Nandi S and Gupta PSP. 2015. Effect of fibroblast growth factor 2 (FGF2) and insulin transferrin selenium (ITS) on in vitro maturation, fertilization and embryo development in sheep. Brazilian Archives of Biology and Technology, 58:521-525.
1516-8913 http://krishi.icar.gov.in/jspui/handle/123456789/12884 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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