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Effect of fibroblast growth factor 2 (FGF2) and insulin transferrin selenium (ITS) on in vitro maturation, fertilization and embryo development in sheep

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Title Effect of fibroblast growth factor 2 (FGF2) and insulin transferrin selenium (ITS) on in vitro maturation, fertilization and embryo development in sheep
Not Available
 
Creator Mondal S
Mor A
Reddy IJ
Nandi S
Gupta PSP
 
Subject fibroblast growth factor 2
insulin transferrin selenium
oocyte
embryo
sheep
 
Description Not Available
The present study evaluated the effect of fibroblast growth factor 2 (FGF2) and insulin-transferrin-selenium (ITS) to the in vitro maturation and embryo culture media on ovine oocyte maturation, cleavage and embryo development. Oocytes having more than five layers of unexpanded cumulus cells and granular homogenous ooplasm were cultured into 50 μL droplets of eight different culture systems: (i) TCM-199 (Tissue Culture Medium-199); (ii) TCM-199+10 ng/mL FGF2; (iii) TCM-199+20 ng/mL FGF2; (iv) TCM-199+30 ng/mL FGF2; (v) TCM-199+10 ng/mL ITS; (vi) TCM-199+20 ng/mL ITS; (vii) TCM-199+30 ng/mL ITS and (viii) TCM-199+20 ng/mL ITS+20 ng/mL FGF2 in a CO2 incubator at 38.50C for 24 h. All the oocyte culture media were supplemented with 10% FBS, FSH (10 μg/mL) and gentamicin (50 µg/mL). The maturation rate was assessed based on the degree of expansion of cumulus cells and identifying first polar body extrusion into perivitelline space. The matured oocytes were inseminated with 1 to 2 million spermatozoa/mL in Brackett and Oliphant medium and the cleavage rate was checked after 42-48 h post insemination and further cultured for 6-7 days. Maturation and cleavage rates were significantly higher (P
National Agricultural Science Fund
 
Date 2018-11-23T03:07:17Z
2018-11-23T03:07:17Z
2015-01-01
 
Type Research Paper
 
Identifier Mondal S, Mor A, Reddy IJ, Nandi S and Gupta PSP. 2015. Effect of fibroblast growth factor 2 (FGF2) and insulin transferrin selenium (ITS) on in vitro maturation, fertilization and embryo development in sheep. Brazilian Archives of Biology and Technology, 58:521-525.
1516-8913
http://krishi.icar.gov.in/jspui/handle/123456789/12884
 
Language English
 
Relation Not Available;
 
Publisher Not Available