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Role of amino acids as additives on sperm motility, plasma membrane integrity and lipid peroxidation levels at refreeze and post–thawed ram semen

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Title Role of amino acids as additives on sperm motility, plasma membrane integrity and lipid peroxidation levels at refreeze and post–thawed ram semen
Not Available
 
Creator Sangeeta S
Arangasamy A
Kulkarni S
Selvaraju S
 
Subject Amino acids
Freezing
HOS-G
Lipid peroxidation
Ram semen
 
Description Not Available
The possibility of including amino acids for cryopreservation of ram semen to improve the quality of frozen semen was explored in this study in sheep model. 24 samples were collected in triplicate from 8 rams of 2-3 year old Bannur cross bred rams maintained at the Institute Experimental Livestock Unit. Semen was diluted in tris-egg yolk glycerol diluent and made into 7 aliquots as follows: aliquot 1 served as control, "l-alanine" was added at 100 and 135mM in the aliquots 2 and 3, "l-glutamine" was added at 20 and 25mM in the aliquots 4 and 5 and "l-proline" was added at 25 and 50mM in the aliquots 6 and 7, respectively. Diluted semen was filled in 0.25ml French straws and frozen in LN2. Inclusion of "l-proline" and "l-glutamine" in the diluent increased the percent live sperm (P
Not Available
 
Date 2018-11-23T08:18:18Z
2018-11-23T08:18:18Z
2015-10-01
 
Type Research Paper
 
Identifier Sangeeta S, Arangasamy A, Kulkarni S and Selvaraju S. 2015. Role of amino acids as additives on sperm motility, plasma membrane integrity and lipid peroxidation levels at refreeze and post–thawed ram semen. Animal Reproduction Science, 161:82-88.
0378-4320
http://krishi.icar.gov.in/jspui/handle/123456789/12953
 
Language English
 
Relation Not Available;
 
Publisher Elsevier