Novel insights into the role of cell free seminal plasma-mRNAs on semen quality and cryotolerance of spermatozoa in bulls (Bos taurus)
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Title |
Novel insights into the role of cell free seminal plasma-mRNAs on semen quality and cryotolerance of spermatozoa in bulls (Bos taurus)
Not Available |
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Creator |
Shilpa M
Selvaraju S Girish Kumar V Parthipan S Binsila KB Arangasamy A Ravindra JP |
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Subject |
cryoinjury
transcript expression |
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Description |
Not Available
The aim of the present study was to ascertain the effectiveness of seminal plasma mRNAs as markers to assess the reproductive performance of bulls. Semen samples (33 ejaculates) from 11 bulls were evaluated for sperm kinematic and functional parameters. Total RNA was isolated from cell-free seminal (cfs) using TRIzol LS reagent and the concentration of cfs-RNA was 24.4±2.3µgmL-1 seminal plasma. The cfs-RNA was fragmented to a size of 25-500bp. Of the cfs-mRNAs screened using real time PCR, expression of protamine 1 (PRM1) was positively (P85%) in raw semen was positively (P Not Available |
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Date |
2018-11-22T05:16:41Z
2018-11-22T05:16:41Z 2017-06-14 |
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Type |
Research Paper
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Identifier |
Shilpa M, Selvaraju S, Girish Kumar V, Parthipan S, Binsila KB, Arangasamy A and Ravindra JP. 2017. Novel insights into the role of cell free seminal plasma-mRNAs on semen quality and cryotolerance of spermatozoa in bulls (Bos taurus). Reproduction Fertility and Development, 29:2446-2456.
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/12427 |
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Language |
English
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Relation |
Not Available;
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Publisher |
CSIRO Publishing
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