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Novel insights into the role of cell free seminal plasma-mRNAs on semen quality and cryotolerance of spermatozoa in bulls (Bos taurus)

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Title Novel insights into the role of cell free seminal plasma-mRNAs on semen quality and cryotolerance of spermatozoa in bulls (Bos taurus)
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Creator Shilpa M
Selvaraju S
Girish Kumar V
Parthipan S
Binsila KB
Arangasamy A
Ravindra JP
 
Subject cryoinjury
transcript expression
 
Description Not Available
The aim of the present study was to ascertain the effectiveness of seminal plasma mRNAs as markers to assess the reproductive performance of bulls. Semen samples (33 ejaculates) from 11 bulls were evaluated for sperm kinematic and functional parameters. Total RNA was isolated from cell-free seminal (cfs) using TRIzol LS reagent and the concentration of cfs-RNA was 24.4±2.3µgmL-1 seminal plasma. The cfs-RNA was fragmented to a size of 25-500bp. Of the cfs-mRNAs screened using real time PCR, expression of protamine 1 (PRM1) was positively (P85%) in raw semen was positively (P
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Date 2018-11-22T05:16:41Z
2018-11-22T05:16:41Z
2017-06-14
 
Type Research Paper
 
Identifier Shilpa M, Selvaraju S, Girish Kumar V, Parthipan S, Binsila KB, Arangasamy A and Ravindra JP. 2017. Novel insights into the role of cell free seminal plasma-mRNAs on semen quality and cryotolerance of spermatozoa in bulls (Bos taurus). Reproduction Fertility and Development, 29:2446-2456.
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http://krishi.icar.gov.in/jspui/handle/123456789/12427
 
Language English
 
Relation Not Available;
 
Publisher CSIRO Publishing