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Epidemiological and molecular characterization of Brucella species in cattle

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Title Epidemiological and molecular characterization of Brucella species in cattle
Not Available
 
Creator Raghava S
Honnayakanahalli M
Gowda M
Shome R
Kulkarni M
Umesha S
 
Subject Brucellosis
infected cattle
PCR
PCR- SSCP
PCR-LSSP
 
Description Not Available
Background: Bovine brucellosis is a severe threat to livestock and mankind as it is a food-borne and occupational zoonosis. Rapid
transmission, high morbidity and mortality are the main features of zoonotic diseases, leading to great personal and economic losses
within a short period of time. Therefore, this study aimed for prompt identification and characterization of Brucella species in livestock
to control the spread of infection and epidemiological data for the planning of disease control strategies is required. Methodology: Five
hundred milk and blood samples were collected from cattle from different regions of Karnataka. All milk and blood samples were
examined by Milk Ring Test (MRT) and Rose Bengal Test (RBT) to detect Brucella antibodies, polymerase chain reaction to detect Brucella
specific DNA. Low-stringency Single Specific Primer Polymerase Chain Reaction (LSSP-PCR) gene signatures and Single-Strand Chain
Polymorphism Polymerase Chain Reaction (SSCP-PCR) were used to study polymorphic variations of Brucella species. Results: Amongst
a total of 500 blood and 500 milk samples, 4.6% prevalence of brucellosis was found in Karnataka. The PCR assay was affirmative with the
Rose Bengal Test (RBT) (4.6%) and Milk Ring Test (MRT) which yielded (3.4%) lower prevalence. The positive samples were confirmed as
Brucella abortus by Bruce-ladder multiplex PCR. The LSSP-PCR, SSCP-PCR gene signatures showed high genetic similarity and
intraspecific similarity which are reproducible. Conclusion: Symptoms of brucellosis are not pathognomonic, diagnosis rely mostly
on the laboratory tests. Hence, the SSCP-PCR, LSSP-PCR gene signatures can be used as an alternative for detection of brucellosis,
screening a large number of clinical samples and identify epidemiological diversity. It also minimizes the drawback of cross-reactivity and
only suspected mutants can be sequenced.
Not Available
 
Date 2018-11-12T05:07:14Z
2018-11-12T05:07:14Z
2017-04-15
 
Type Research Paper
 
Identifier Not Available
1819-1878
http://krishi.icar.gov.in/jspui/handle/123456789/10256
 
Language English
 
Relation Not Available;
 
Publisher Asian Journal of Animal Sciences