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Uncleaved 2A-peptide of foot-and-mouth disease virus can display foreign epitope-tag at the virion surface

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Title Uncleaved 2A-peptide of foot-and-mouth disease virus can display foreign epitope-tag at the virion surface
Not Available
 
Creator Biswal JK, Subramaniam S, Ranjan R, Pattnaik B
 
Subject FMD virus, Uncleaved 2A, peptide HA, epitope tag, Reverse genetics,Tagged FMDV
 
Description Not Available
Foot-and-mouth disease virus (FMDV) capsid precursor protein P1-2A is cleaved by viral-encoded 3C protease (3Cpro) to generate VP0, VP3, VP1 and 2A proteins. It was reported earlier that substitution of a single amino acid residue within the 2A peptide sequence (L2P) blocked the 3Cpro mediated VP1/2A cleavage and produced ‘self-tagged’ FMDV particles containing uncleaved 2A-peptide. To determine whether the uncleaved 2A-peptide can function as a target structure to harbour and display exogenous epitope on FMDV particles, a full-length FMDV cDNA clone containing a HA-tag within the uncleaved 2A-peptide sequence was constructed. Subsequently, chimeric marker FMDV, displaying a HA-tag on the viral surface was rescued through reverse genetics approach. The 2A-HA epitope tag-inserted recombinant chimeric FMDV serotype O was genetically stable through up to ten serial passages in cell culture and exhibited growth properties similar to the parental virus. Furthermore the surface displayed HA-epitope tag was able to react with anti-HA antibodies as determined by various immuno-assays. The results from our study suggest that the uncleaved 2A-peptide of FMDV is suitable to present foreign antigenic epitopes on the surface of FMD virion.
ICAR
 
Date 2018-11-26T13:50:40Z
2018-11-26T13:50:40Z
2017-07-19
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/13487
 
Language English
 
Relation Not Available;
 
Publisher Elsevier