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Parasitological and molecular detection of bovine babesiosis in endemic areas of Karnataka state.

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Title Parasitological and molecular detection of bovine babesiosis in endemic areas of Karnataka state.
Not Available
 
Creator Vijaykumar K U
Puttalakshamma G C
D’Souza P E
Sengupta P P
Chandranaik B M
Renukaprasad C
 
Subject Acridine orange staining
Bovine babesiosis
Babesia bigemina
Giemsa
Fluorescent
PCR
 
Description Not Available
A study was conducted to compare the specificity and sensitivity of parasitological methods (Giemsa and
Fluorescent acridine orange staining) and PCR to detect the carrier status, subclinical and clinical babesiosis in
cattle from endemic regions of Karnataka state in South India. Out of 148 cattle screened, 3 (2.03%), 7 (4.73%) and
16 (10.81%) were found positive for B. bigemina infection by Giemsa’s staining, acridine orange staining and
PCR, respectively. The conserved primers for B. bigemina derived from SpeI-AvaI restriction fragment were used
in PCR which amplified a fragment of 278 bp in the positive cattle blood and was 83.26% sensitive and 91.16%
specific for detection of babesiosis in cattle.
Not Available
 
Date 2018-11-12T04:22:50Z
2018-11-12T04:22:50Z
2017-04-01
 
Type Research Paper
 
Identifier Not Available
2231-0916
http://krishi.icar.gov.in/jspui/handle/123456789/10206
 
Language English
 
Relation Not Available;
 
Publisher Indian Council of Agricultural Research