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Comparative evaluation of blood based lateral flow assay for diagnosis of brucellosis in livestock species

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Title Comparative evaluation of blood based lateral flow assay for diagnosis of brucellosis in livestock species
Not Available
 
Creator Kavya B A
Veeregowda B M
Kamran A
Gomes A R
Triveni K
Padmashree B S
Shome R
 
Subject Brucellosis
Blood-LFA
RBPT
Serum-LFA
 
Description Not Available
Brucellosis is a zoonotic and economically important disease of livestock. Pen side diagnostics are the need of
the hour for the rapid diagnosis of brucellosis. The present study aimed to evaluate blood based lateral flow assay
(LFA) with serum based LFA and rose bengal plate test (RBPT) for diagnosis of brucellosis. Sera/blood samples
[792: cattle, 153; buffalo, 55; sheep, 140; goats, 219; and pigs, 225] were collected from Karnataka, India with
history of repeat breeding, retention of placenta and abortion.The seropositivity of brucellosis in cattle, buffalo,
sheep, goats and pigs was 7.84, 3.63, 12.85, 2.73 and 39.11% by RBPT; 5.88, 3.63, 8.57, 2.73 and 31.1% by blood-
LFA; and 7.18, 3.63, 10.71, 3.19 and 32.88% by serum-LFA. Relative specificity of blood based LFA was between
98.97 to 99.53% and 97.42 to 100% in comparison to serum-LFA and RBPT, respectively in all the species. The
relative sensitivity for all the species ranged from 64.29 to 83.33% by blood-LFA and 57.14 to 84.09% by serum-
LFA. The diagnostic accuracy of blood-LFA to RBPT and serum- LFA ranged from 91 to 99% among the species
studied. It is opined that blood based LFA can serve as diagnostic test in place of serum based LFA for brucellosis
screening in multiple livestock species and suggested for adaptation on-farm, slaughter, market, clinical and prepurchase
surveillance of brucellosis in the country.
Not Available
 
Date 2018-11-12T05:16:59Z
2018-11-12T05:16:59Z
2017-09-01
 
Type Research Paper
 
Identifier Not Available
2157-7579
http://krishi.icar.gov.in/jspui/handle/123456789/10276
 
Language English
 
Relation Not Available;
 
Publisher Indian Council of Agricultural Research