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Hope for restoration of dead valuable bulls through cloning using donor somatic cells isolated from cryopreserved semen

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Title Hope for restoration of dead valuable bulls through cloning using donor somatic cells isolated from cryopreserved semen
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Creator Naresh L Selokar, Monika Saini, Prabhat Palta, Manmohan S Chauhan, Radheysham Manik, Suresh K Singla
 
Subject Buffalo, SCNT, Embryo, Bull
 
Description Not Available
Somatic cells were isolated from cryopreserved semen of 4 buffalo bulls, 3 of which had died over 10 years earlier, and were established in culture. The cells expressed cytokeratin-18, keratin and vimentin indicating that they were of epithelial origin. The cells were used as nuclear donors for hand-made cloning for producing buffalo embryos. The blastocyst rate and quality, as indicated by apoptotic index, were comparable among embryos produced using cells obtained from fresh or frozen-thawed semen or those obtained from conventional cell sources such as skin. Examination of the epigenetic status revealed that the global level of H3K27me3 but not that of H3K9/14ac and H4K5ac differed significantly (P
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Date 2018-11-20T04:13:28Z
2018-11-20T04:13:28Z
2014-03-10
 
Type Article
 
Identifier 29
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/12164
 
Language English
 
Relation Not Available;
 
Publisher Public Library of Science