Hope for restoration of dead valuable bulls through cloning using donor somatic cells isolated from cryopreserved semen
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Title |
Hope for restoration of dead valuable bulls through cloning using donor somatic cells isolated from cryopreserved semen
Not Available |
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Creator |
Naresh L Selokar, Monika Saini, Prabhat Palta, Manmohan S Chauhan, Radheysham Manik, Suresh K Singla
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Subject |
Buffalo, SCNT, Embryo, Bull
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Description |
Not Available
Somatic cells were isolated from cryopreserved semen of 4 buffalo bulls, 3 of which had died over 10 years earlier, and were established in culture. The cells expressed cytokeratin-18, keratin and vimentin indicating that they were of epithelial origin. The cells were used as nuclear donors for hand-made cloning for producing buffalo embryos. The blastocyst rate and quality, as indicated by apoptotic index, were comparable among embryos produced using cells obtained from fresh or frozen-thawed semen or those obtained from conventional cell sources such as skin. Examination of the epigenetic status revealed that the global level of H3K27me3 but not that of H3K9/14ac and H4K5ac differed significantly (P Not Available |
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Date |
2018-11-20T04:13:28Z
2018-11-20T04:13:28Z 2014-03-10 |
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Type |
Article
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Identifier |
29
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/12164 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Public Library of Science
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