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Seminal plasma protein profiles in three different breeds of equines

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Title Seminal plasma protein profiles in three different breeds of equines
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Creator THIRUMALA RAO TALLURI, MAL GORAKH, S K RAVI, PAL Y, PATIL N V, SINGH R K
 
Subject Exotic jack, Marwari stallion, Stallion seminal plasma protein, Total protein, Zanskari stallion
 
Description Not Available
Semen ejaculates (8) were obtained from 2 Marwari stallions, 2 Zanskari stallions and 2 exotic jacks (martina
franca). Gel portion of the semen was removed by filtration and immediately the seminal plasma was separated by
centrifugation at 2000 rpm for 30 min and stored at –20°C until further analysis. Total protein from the seminal plasma
was estimated by Lowry method and the molecular weight was analysed by SDS-PAGE. The total protein concentration
was found to be 0.71, 2.24 and 2.21 g/dl in Marwari, Zanskari and exotic jack seminal plasma respectively. The differences
in protein concentration were found to be significantly higher in Zanskari and jack stallions as compared to Marwari
stallion. There was no significant difference of total protein values between Zanskari and jack stallions. A total of 12
protein bands were observed in the seminal plasma of both Marwari and Zanskari stallions and are in common, whereas,
15 protein bands were observed in the seminal plasma of exotic jacks. The seminal plasma proteins in Marwari and
Zanskari stallions ranged from 11.45KDa to 130.23kDa and that of exotic jack ranged from 11.45kDa to 162.83kDa.
Out of 15 protein bands observed, 3 were unique in jack stallions having molecular weight of 36.52 kDa, 39.25 kDa and
1602.83 kDa. It can be concluded from this study that at least 12 bands of protein were in common between the all the
3 breeds of equines, and seminal plasma protein profile of jack stallion is different from the Marwari and Zanskari
stallions.
ICAR
 
Date 2018-11-20T07:05:52Z
2018-11-20T07:05:52Z
2012-04-01
 
Type Article
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/12297
 
Language English
 
Relation Not Available;
 
Publisher Indian Journal of Animal Sciences