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Isolation and culture of putative mesenchymal stem cells from equine umbilical cord Wharton’s jelly

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Title Isolation and culture of putative mesenchymal stem cells from equine umbilical cord Wharton’s jelly
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Creator N S RATHORE, S K KASHYAP, ANUPAMA DEORA, PANKAJ KUMAR, J SINGH, B N TRIPATHI, T R TALLURI
 
Subject Collagenase, Equine, Mesenchymal stem cells, Tissue explants, Trypsin, Umbilical cord, Wharton’s jelly
 
Description Not Available
Despite major progress and knowledge related to the application of adult stem cells, finding alternative sources
for bone marrow MSCs has remained a challenge in both humans and animals. In the current study, two protocols
namely sequential enzymatic tissue digestion and tissue explant techniques were tried for successful establishment
of MSC culture. Umbilical tissues were isolated each time of foaling from five sequential foalings of Marwari
mares. Total cell yield, their growth potential and cryopreservation potential were studied. Adherent cell colonies
could be established using both isolation methods. Both the cell populations yielded from different protocols
performed similarly in terms of population doubling and CFU number value. Additionally, the cells proliferated
vigourously and displayed a similar morphology of mesenchymal stem cells. The MSCs were plastic adherent,
colonogenic and their morphology was polygonal and fibroblast like. During the proliferation, the cells exhibited
density dependent inhibition; analysis of microbial contamination from bacteria, mycoplasma and fungi were
negative; the population doubling time of the MSCs isolated was 34.8 h and 40.2 h in enzymatic treatment and
tissue explant methods respectively, and diploid chromosome number of the cells was 64, and the diploid frequency
was higher than 80%. In conclusion, this study reveals that both the techniques proved to be non-invasive, efficient,
simple and quick for isolation and establishment of MSC culture of extra embryonic tissues from equines.
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Date 2018-11-20T07:06:02Z
2018-11-20T07:06:02Z
2018-09-01
 
Type Article
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/12298
 
Language English
 
Relation Not Available;
 
Publisher Indian Journal of Animal Sciences 88 (9): 1025–1029, September 2018/Article