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Cryosurvival of Marwari stallion sperm in different extenders

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Title Cryosurvival of Marwari stallion sperm in different extenders
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Creator TEJPAL TEJPAL, J S MEHTA, S K RAVI, T R TALLURI, ASHOK KUMAR, AMIT KUMAR, YOGESH SONI
 
Subject Cryopreservation, Extender, Marwari horse, Semen
 
Description Not Available
Extenders (3) were compared for frozen-thawed semen quality of Marwari horses in the present study. Ejaculates
(36) including 6 ejaculates from each of 6 stallions were collected using artificial vagina (AV) method. The ejaculates
were cryopreserved either with glucose-EDTA-lactose, HF-20 or egg yolk-skim milk extender using custom freezing
technique. Sperm characteristics, viz. motility, viability, acrosome intactness and plasma membrane integrity were
studied in frozen-thawed semen besides record of fresh semen quality as a prerequisite. Present study indicated a
non-significant difference in frozen-thawed semen parameters with glucose-EDTA-lactose and HF-20 extenders,
however, both the extenders resulted in a higher percentage of post-thaw motility (PTM), viability, intact acrosome
and plasma membrane integrity of sperm than the egg yolk-skim milk extender. It was concluded that glucoseEDTA-lactose
and HF-20 are better semen extenders as compared to egg yolk-skim milk and they can be used
effectively for semen cryopreservation in horses.
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Date 2018-11-20T07:06:13Z
2018-11-20T07:06:13Z
2016-12-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/12299
 
Language English
 
Relation Not Available;
 
Publisher Indian Journal of Animal Sciences 86 (12): 1396–1400, December 2016/Article