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Diagnostic application of recombinant equine merozoite surface antigen-1 in elisa for detection of Theileria equi specific antibodies

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Title Diagnostic application of recombinant equine merozoite surface antigen-1 in elisa for detection of Theileria equi specific antibodies
Not Available
 
Creator Sandeep Kumar, Naresh K. Rakha, Liza Goyal, Parveen Goel, Rajender Kumar, Ashok Kumar and Sanjay Kumar
 
Subject Theileria equi, EMA-1, diagnosis, ELISA, Babesia equi.
 
Description Not Available
Theileria equi merozoite surface antigens have been an important candidate for
development of diagnostics. We developed ELISA based on EMA-1 recombinant antigen, so
as to widen our diagnostic confidence in detection of antibodies against T. equi in serosurveillance
studies. The 547 bp EMA-1 gene fragment encoding high hydrophilic antigenic
region was expressed with glutathione-S-transferase tag in prokaryotic system and purified
protein (43 kDa) was used for development of ELISA (EMA-1t/ELISA). The EMA-1t/ELISA
clearly differentiated T. equi-infected from Babesia caballi-infected horse sera or normal
horse sera. The results of the study were validated with previously developed EMA-2ELISA on
serum samples of known T. equi infection status and a very high correlation (0.93) was
recorded between the relative percent positivity (RPP) values. Further diagnostic
sensitivity of EMA-1t/ELISA was 0.92 while specificity was 1.0, indicating its suitability for
sero-epidemiological studies. This assay was applied on serum samples (n ＝ 240) collected
from field horses in northern part of India. High sero-prevalence of T. equi antibodies were
diagnosed in serum samples collected from Haryana state (74%) and Uttarakhand state
(36.31%). Results of this study suggested that the 43 kDa EMA-1 expressed protein could be
a reliable immunodiagnostic antigen in ELISA for T. equi sero-prevalence studies.
Not Available
 
Date 2018-11-20T11:04:01Z
2018-11-20T11:04:01Z
2015-07-06
 
Type Journal
 
Identifier Not Available
0047-1917
http://krishi.icar.gov.in/jspui/handle/123456789/12354
 
Language English
 
Relation Not Available;
 
Publisher Japanese Journal of Veterinary Research