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Immunokinetics of Theileria equi specific antibodies: a comparison in serial and single dilution ELISA antibody end titres

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Title Immunokinetics of Theileria equi specific antibodies: a comparison in serial and single dilution ELISA antibody end titres
Not Available
 
Creator Yogesh KUMAR, Dharam Vir MALHOTRA, Anil Kumar NICHANI, Ashok KUMAR, Shruti DHAR, Sanjay KUMAR
 
Subject Babesia equi, ELISA, equine piroplasmosis, immunokinetics, Theileria equi
 
Description Not Available
Theileria equi, a haemoprotozoan of equids, is responsible for endemic infection/latency in tropical/subtropical parts of the
world. The present study investigated the immunokinetics of T. equi infection in experimental animals and the diagnostic applicability of
single dilution ELISA vis-à-vis conventional serial dilution ELISA. Sequential serum samples were collected up to 90 days postinfection
(PI) from 4 donkeys experimentally infected with T. equi. Immunokinetics of T. equi antibodies end titre were determined by serial
and single dilution ELISA. A significant rise in initial ELISA antibody end titre (1:800 in serial dilution ELISA and from 1:600 to 1:650
in single dilution ELISA) was observed from day 9 PI in all 4 donkeys, which plateaued between days 66 and 73 PI (end antibody titre
varied between 1:6400 to 1:25,600 and 1:6050 to 1:22,100). The efficacy of these ELISAs was also determined by testing 256 field serum
samples at 1:200 dilution, collected from apparently healthy animals. Sample analysis revealed overall seroprevalence of 91.01% in the
horse population. The frequency distribution of antibody titre of these field samples by single dilution ELISA exhibited that most of the
positive horses had high levels of specific antibodies. This study has emphasised the usefulness of single dilution ELISA as a diagnostic
assay in determining the end antibody titre by testing it at a single dilution of 1:200.
Not Available
 
Date 2018-11-20T11:03:07Z
2018-11-20T11:03:07Z
2013-07-29
 
Type Journal
 
Identifier Not Available
1300-0128
http://krishi.icar.gov.in/jspui/handle/123456789/12349
 
Language English
 
Relation Not Available;
 
Publisher TUBITAK