Development of EMA-2 recombinant antigen based enzyme-linked immunosorbent assay for seroprevalence studies of Theileria equi infection in Indian equine population
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Title |
Development of EMA-2 recombinant antigen based enzyme-linked immunosorbent assay for seroprevalence studies of Theileria equi infection in Indian equine population
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Creator |
Sanjay Kumar, Rajender Kumar, Ashok K. Gupta, Suresh C. Yadav, Sachin K. Goyal, Sandip K. Khurana, Raj K. Singh
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Subject |
Babesia equi ,Equine piroplasmosis,ELISA ,EMA-2 ,Seroprevalence ,Theileria equi
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Description |
Not Available
Equine piroplasmosis is a tick-transmitted protozoan disease caused by Theileria equi and/or Babesia caballi. In the present study, we expressed a 53 kDa protein from the truncated EMA- 2 gene of T. equi (Indian strain) and developed EMA-2ELISA using this expressed protein. This ELISA is able to detect T. equi-specific antibodies in experimentally infected animals as early as 9 days post-infection. The assay developed was validated with the OIE recommended competitive ELISA (cELISA) on 120 serum samples and significant agreement (kappa = 0.93) was observed between results of both the ELISAs which indicates suitability of EMA-2ELISA for use in sero-diagnosis. Diagnostic sensitivity and specificity of EMA-2ELISA – as compared with cELISA – were 0.97 and 0.96, respectively. Analysis of 5651 equine serum samples – collected during 2007–2012 from 12 states of India representing eight agro-climatic zones – by EMA-2ELISA revealed 32.65% seroprevalence of T. equi in India. In conclusion, the EMA-2ELISA developed using the T. equi EMA-2 recombinant protein as antigen for detecting T. equi-specific antibodies has good diagnostic potential for sero-epidemiological surveys. Not Available |
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Date |
2018-11-20T11:03:17Z
2018-11-20T11:03:17Z 2013-08-30 |
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Type |
Journal
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Identifier |
Not Available
0304-4017 http://krishi.icar.gov.in/jspui/handle/123456789/12350 |
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Language |
English
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Relation |
Not Available;
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Publisher |
ELSEVIER
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