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Development of EMA-2 recombinant antigen based enzyme-linked immunosorbent assay for seroprevalence studies of Theileria equi infection in Indian equine population

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Title Development of EMA-2 recombinant antigen based enzyme-linked immunosorbent assay for seroprevalence studies of Theileria equi infection in Indian equine population
Not Available
 
Creator Sanjay Kumar, Rajender Kumar, Ashok K. Gupta, Suresh C. Yadav, Sachin K. Goyal, Sandip K. Khurana, Raj K. Singh
 
Subject Babesia equi ,Equine piroplasmosis,ELISA ,EMA-2 ,Seroprevalence ,Theileria equi
 
Description Not Available
Equine piroplasmosis is a tick-transmitted protozoan disease caused by Theileria equi and/or
Babesia caballi. In the present study, we expressed a 53 kDa protein from the truncated EMA-
2 gene of T. equi (Indian strain) and developed EMA-2ELISA using this expressed protein. This
ELISA is able to detect T. equi-specific antibodies in experimentally infected animals as early
as 9 days post-infection. The assay developed was validated with the OIE recommended
competitive ELISA (cELISA) on 120 serum samples and significant agreement (kappa = 0.93)
was observed between results of both the ELISAs which indicates suitability of EMA-2ELISA
for use in sero-diagnosis. Diagnostic sensitivity and specificity of EMA-2ELISA – as compared
with cELISA – were 0.97 and 0.96, respectively. Analysis of 5651 equine serum samples
– collected during 2007–2012 from 12 states of India representing eight agro-climatic
zones – by EMA-2ELISA revealed 32.65% seroprevalence of T. equi in India. In conclusion, the
EMA-2ELISA developed using the T. equi EMA-2 recombinant protein as antigen for detecting
T. equi-specific antibodies has good diagnostic potential for sero-epidemiological surveys.
Not Available
 
Date 2018-11-20T11:03:17Z
2018-11-20T11:03:17Z
2013-08-30
 
Type Journal
 
Identifier Not Available
0304-4017
http://krishi.icar.gov.in/jspui/handle/123456789/12350
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER