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Regulation of iron-siderophore uptake in a diazotrophic cyanobacterium Anabaena anabaena 7120

Indian Agricultural Research Journals

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Title Regulation of iron-siderophore uptake in a diazotrophic cyanobacterium Anabaena anabaena 7120
 
Creator SAXENA, RISHI KUMAR
YADAV, RAVINDRA K
SINGH, SURENDRA
 
Subject Anabaena 7120, Cyanobacterium, , Iron- siderophore uptake
 
Description The regulation of 55Fe-siderophore uptake of was studied in a diazotrophic cyanobacterium Anabaena 7120. The uptake up to 20 min was found to be biphasic, a rapid first and steady second phase. A single kinetic system mediated the uptake of iron-siderophore with an apparent Km of 200 µM and a Vmax of 20 nmol iron taken up mg-1 protein min-1. 3-(3,4dichlorophenyl)-1, 1-dimethyl urea (DCMU), 2-n-heptyl-4-hydroxy quinoline (HOQNO), dicyclohexyl carbodiimide (DCCD), carbonyl cyanide p-fluoromethoxy phenyl hydrazone (FCCP) and carbonyl cyanide-m-chlorophenyl hydrazone (CCCP) inhibited the iron-siderophore uptake significantly, whereas KCN inhibited marginally. No iron-siderophore uptake was observed under dark-aerobic and dark-anaerobic conditions. The siderophore schizokinen supported the maximum iron-siderophore uptake. However, the siderophore aerobactin and desferal could support only 53 and 3.4% iron-siderophore uptake, respectively as compared to its schizokinen counterpart.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2014-01-28
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/37158
 
Source The Indian Journal of Agricultural Sciences; Vol 84, No 1 (2014)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/37158/16598
 
Rights Copyright (c) 2014 The Indian Journal of Agricultural Sciences