Genetic differentiation amongst different Trypanosoma evansi isolates using random primer DNA typing
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Title |
Genetic differentiation amongst different Trypanosoma evansi isolates using random primer DNA typing
Not Available |
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Creator |
RAJENDER KUMAR, PARVATI SHARMA, DEEPAK KUMAR GAUR, SACHIN KUMAR GOYAL and SANJAY KUMAR
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Subject |
DNA, Genetic variability, RAPD, Surra, Trypanosoma evansi
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Description |
Not Available
In order to know the genetic variability amongst the 6 isolates of Trypanosoma evansi obtained from different livestock species (horse, donkey, camel and cattle) of different geographical regions, genomic DNA was amplified by polymerase chain reaction using 17 decamer arbitrary primers. In PCR profiles, 13 out of the 17 primers generated differences. The primer PR2 showed different polymorphic DNA fragments among different isolates of T. evansi which were unique for particular isolate and all these band were scorable as either distinct or likely distinct. Various informative bands ranging from 250 to 4,000 bp were observed through PCR amplified DNA profile of different isolates and marked the minor and major difference among the isolates. Randomly amplified polymorphic DNA (RAPD) analysis revealed heterogeneity among T. evansi isolates of different livestock hosts and geographical regions. Not Available |
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Date |
2018-11-22T06:14:32Z
2018-11-22T06:14:32Z 2015-04-22 |
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Type |
Journal
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Identifier |
Not Available
03678318 http://krishi.icar.gov.in/jspui/handle/123456789/12551 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Indian Journal of Animal Sciences
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