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Genetic differentiation amongst different Trypanosoma evansi isolates using random primer DNA typing

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Title Genetic differentiation amongst different Trypanosoma evansi isolates using random primer DNA typing
Not Available
 
Creator RAJENDER KUMAR, PARVATI SHARMA, DEEPAK KUMAR GAUR, SACHIN KUMAR GOYAL and SANJAY KUMAR
 
Subject DNA, Genetic variability, RAPD, Surra, Trypanosoma evansi
 
Description Not Available
In order to know the genetic variability amongst the 6
isolates of Trypanosoma evansi obtained from different
livestock species (horse, donkey, camel and cattle) of
different geographical regions, genomic DNA was amplified
by polymerase chain reaction using 17 decamer arbitrary
primers. In PCR profiles, 13 out of the 17 primers generated
differences. The primer PR2 showed different polymorphic
DNA fragments among different isolates of T. evansi which
were unique for particular isolate and all these band were
scorable as either distinct or likely distinct. Various
informative bands ranging from 250 to 4,000 bp were
observed through PCR amplified DNA profile of different
isolates and marked the minor and major difference among
the isolates. Randomly amplified polymorphic DNA (RAPD)
analysis revealed heterogeneity among T. evansi isolates of
different livestock hosts and geographical regions.
Not Available
 
Date 2018-11-22T06:14:32Z
2018-11-22T06:14:32Z
2015-04-22
 
Type Journal
 
Identifier Not Available
03678318
http://krishi.icar.gov.in/jspui/handle/123456789/12551
 
Language English
 
Relation Not Available;
 
Publisher Indian Journal of Animal Sciences