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Quantification of Trypanosoma evansi parasitic load in experimentally infected mice using real time PCR assay and its comparative evaluation with conventional parasitological techniques

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Title Quantification of Trypanosoma evansi parasitic load in experimentally infected mice using real time PCR assay and its comparative evaluation with conventional parasitological techniques
Not Available
 
Creator SHASHI KANT KANKAR, RAJENDER KUMAR, SACHIN KUMAR GOYAL, DEEPAK KUMAR GAUR, PARVATI SHARMA, B C BERA, RAJAT GARG, SANJAY KUMARand S C YADAV
 
Subject ITS-1, Molecular diagnosis, PCR, qPCR, Surra, SYBER, Trypanosoma evansi
 
Description Not Available
Trypanosoma evansi causes an economically important disease called surra, which is responsible for significant
losses to livestock productivity in Asia, Africa, Central and South America. In the present study, a SYBR Green
based quantitative PCR (qPCR) assay was optimized using primers targeting the internal transcribed spacer 1
(ITS–1) region of rRNA gene for quantitative estimation of T. evansi infection in experimentally infected mice.
The sensitivity of qPCR assay was found approximate to the gold standard TBR1/2 primers based PCR assay and
two times more than the conventional parasitological techniques, viz. wet blood film (WBF) stained thin blood
smear (TBS) and microhaematocrit centrifugation test (MHCT). The detection limit of the assay was 1.5 parasite
equivalence or 0.15 pg T. evansi DNA. On fourth day post infection, 100% (6/6) mice were detected positive by all
parasitological techniques with parasitaemia ranging from 1.6×105 to 2.1×108 parasites. The study indicated that
this assay can be applied for quantitative estimation of parasitaemia in animals suffering from surra and may be
helpful for understanding disease status, risk analysis and efficacy of drug used in treatment of disease.
Not Available
 
Date 2018-11-22T06:14:52Z
2018-11-22T06:14:52Z
2016-05-23
 
Type Journal
 
Identifier Not Available
03678318
http://krishi.icar.gov.in/jspui/handle/123456789/12555
 
Language English
 
Relation Not Available;
 
Publisher Indian Journal of Animal Sciences