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Characterization of Mithun (Bos frontalis) ejaculates and fertility of cryopreserved sperm

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Title Characterization of Mithun (Bos frontalis) ejaculates and fertility of cryopreserved sperm
Not Available
 
Creator Not Available
Mohan Mondal
M. Karunakaran
Kyung-Bon Lee
Chandan Rajkhowa
 
Subject Mithun (Bos frontalis) Semen Cryopreservation Conservation Fertility Artificial insemination
 
Description Not Available
Technologies for conservation and propagation of genetic resources in the Mithun (Bos
frontalis), a rare semi-wild bovine species of Southeast Asia. Successful cryopreservation
of Mithun semen would provide a potential vehicle to address above issue. To date, information
on characteristics of Mithun ejaculates is not available and there are no reports
of birth of live offspring using cryopreserved Mithun semen collected using AV method.
A study was therefore conducted to (i) characterize the Mithun ejaculate, (ii) investigate
the effectiveness of Mithun sperm cryopreservation, and (iii) determine whether artificial
insemination using frozen–thawed Mithun sperm can result in live offspring. Semen
samples collected from eight fertile Mithun bulls were evaluated for colour, consistency,
volume, concentration, mass activity and progressive motility. The freshly ejaculated sperm
were also evaluated for morphological abnormalities, live sperm counts, acrosome integrity,
membrane stability (hypo-osmotic swelling test; HOST) and DNA integrity. Semen samples
of good quality were cryopreserved in liquid nitrogen using Tris–egg yolk–glycerol
diluent. Post-thaw quality of the cryopreserved sperm in terms of progressive motility,
morphological abnormalities, live sperm counts, acrosome integrity, membrane stability
and DNA integrity were assessed. In addition, 16 Mithun cows at estrus were inseminated
with frozen–thawed Mithun sperm. Following cryopreservation, the percentage of
progressive motility (fresh versus frozen–thawed), live sperm counts, morphological abnormalities,
acrosome integrity, membrane stability and DNA integrity were found to decrease
(P
Not Available
 
Date 2019-02-01T06:18:57Z
2019-02-01T06:18:57Z
2009-09-23
 
Type Research Paper
 
Identifier Not Available
0378-4320
http://krishi.icar.gov.in/jspui/handle/123456789/16666
 
Language English
 
Relation Not Available;
 
Publisher Elsevier