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Detection of heat-stable and heat-labile enterotoxin genes of Escherichia coli in diarrhoeic faecal samples of mithun (Bos frontalis) calves by polymerase chain reaction

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Title Detection of heat-stable and heat-labile enterotoxin genes of Escherichia coli in diarrhoeic faecal samples of mithun (Bos frontalis) calves by polymerase chain reaction
Not Available
 
Creator Not Available
S. Rajkhowa
I. Hussain
C. Rajkhowa
 
Subject calves, diarrhoea, LT enterotoxin gene, PCR, ST enterotoxin gene.
 
Description Not Available
Aims: To find out the prevalence of different serogroups of Escherichia coli
(E. coli) and to detect heat-stable (ST) and heat-labile (LT) enterotoxin genes of
enterotoxigenic E. coli (ETEC) from the faeces of mithun calves with diarrhoea.
Methods and Results: Faecal samples obtained from 65 diarrhoeic mithun
calves of under 2 months of age were examined for E. coli using polymerase
chain reaction (PCR). Fifty-four E. coli isolates were obtained from those samples,
which belonged to 38 different serogroups. Out of 54 isolates tested by
PCR, two isolates (3Æ70%) belonging to serogroups O26 and O55 were found
to possess gene that code for ST enterotoxin and one isolate (1Æ85%) belonging
to serogroup O125 was found to carry LT enterotoxin gene.
Conclusions: Escherichia coli isolates from diarrhoeic mithun calves were found
to possess ST and LT enterotoxin genes, which are designated as ETEC, and
these isolates can be detected through PCR using specific primers.
Significance and Impact of the Study: This study reports the isolation of ETEC
possessing ST and LT enterotoxin genes for the first time and ETEC could be a
cause of diarrhoea in mithun calves leading to calf mortality.
Journal of Applied Microbiology ISSN 1364-5072
ª 2009
Not Available
 
Date 2019-02-01T06:19:44Z
2019-02-01T06:19:44Z
2008-07-01
 
Type Research Paper
 
Identifier Not Available
1364-5072
http://krishi.icar.gov.in/jspui/handle/123456789/16669
 
Language English
 
Relation Not Available;
 
Publisher Not Available