Record Details

Non-viral reprogramming of fibroblasts into induced pluripotent stem cells by Sleeping Beauty and piggyBac transposons

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Non-viral reprogramming of fibroblasts into induced pluripotent stem cells by Sleeping Beauty and piggyBac transposons
Not Available
 
Creator Thirumala R. Talluri a,b, Dharmendra Kumar a,c, Silke Glage d, Wiebke Garrels d, Zoltan Ivics e, Katharina Debowski f, Rüdiger Behr f, Wilfried A. Kues a,⇑
 
Subject Pluripotency reporter Transposition Genetic background Inbred Outbred Stemness
 
Description Not Available
The generation of induced pluripotent stem (iPS) cells represents a promising approach for innovative cell
therapies. The original method requires viral transduction of several reprogramming factors, which may
be associated with an increased risk of tumorigenicity. Transposition of reprogramming cassettes represents
a recent alternative to viral approaches. Since binary transposons can be produced as common plasmids
they provide a safe and cost-efficient alternative to viral delivery methods. Here, we compared the
efficiency of two different transposon systems, Sleeping Beauty (SB) and piggyBac (PB), for the generation
of murine iPS. Murine fibroblasts derived from an inbred BL/6 mouse line carrying a pluripotency reporter,
Oct4-EGFP, and fibroblasts derived from outbred NMRI mice were employed for reprogramming. Both
transposon systems resulted in the successful isolation of murine iPS cell lines. The reduction of the core
reprogramming factors to omit the proto-oncogene c-Myc was compatible with iPS cell line derivation,
albeit with reduced reprogramming efficiencies. The transposon-derived iPS cells featured typical hallmarks
of pluripotency, including teratoma growth in immunodeficient mice. Thus SB and PB transposons
represent a promising non-viral approach for iPS cell derivation.
Not Available
 
Date 2019-12-05T11:01:20Z
2019-12-05T11:01:20Z
2014-06-10
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/28163
 
Language English
 
Relation Not Available;
 
Publisher Not Available