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Expression and characterization of recombinant single chain beta-alpha equine chorionic gonadotropin in prokaryotic host

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Title Expression and characterization of recombinant single chain beta-alpha equine chorionic gonadotropin in prokaryotic host
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Creator Anuradha Bhardwaj*, Sanjay Kumar, Varij Nayan1, Parvati Sharma, Yash Pal and S.C. Yadav
 
Subject eCG; Escherichia coli, Expression, Hormone, Pituitary, Protein, Recombinant
 
Description Not Available
The aim of present study was to produce recombinant eCG/pituitary glycoprotein in the Escherichia coli (E. coli) BL21C
host cells and to test its diagnostic efficacy. This aim was achieved by optimizing its expression, purification as well as its
characterization through the immunoassays and bioassays. A bacterial protein expression vector system based on the phage
T7 promoter and histidine tag was used for the expression and purification. The recombinant single chain beta-alpha equine
chorionic gonadotropin (reCG) encoding gene was constructed with beta and alpha sequences according to its biologically
active counterpart. It was successfully cloned and when expressed in E.coli BL21C host, the purified recombinant protein
was found to be active as revealed by enzyme linked immunosorbent assay (ELISA) and Western blotting. However, it was
not found to exhibit any significant activity in vivo when tested in the mice
Not Available
 
Date 2018-11-29T05:13:08Z
2018-11-29T05:13:08Z
2018-03-20
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/13941
 
Language English
 
Relation Not Available;
 
Publisher ARCC