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Lack of Polymorphism in Partial Insulin Like Growth Factor 1 (IGF1) and Insulin Like Growth Factor Binding Protein 3 (IGFBP3) Genes of Mithun

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Title Lack of Polymorphism in Partial Insulin Like Growth Factor 1 (IGF1) and Insulin Like Growth Factor Binding Protein 3 (IGFBP3) Genes of Mithun
Not Available
 
Creator Not Available
Manjit Panigrahi
Subodh Kumar
S. M. Deb
Abhijit Mitra
Arjava Sharma
K. M. Bujarbaruah
 
Subject Bos frontalis, exon 2-3, exon 5, HindIII, IGF-1, IGFBP-3, mithun, NlaIII, PCR-RFLP
 
Description Not Available
Panigrahi, M., Kumar, S., Deb, S.M., Mitra, A., Sharma, A. and Bujarbaruah, K.M. 2009. Lack of
polymorphism in partial insulin like growth factor 1 (IGF1) and insulin like growth factor binding protein
3 (IGFBP3) genes of mithun. J. Appl. Anim. Res., 36: 41-44.
A 396 bp fragment encompassing exon 5 of Insulin like growth factor 1 (IGF1) gene and 652 bp fragment encompassing exon 2-3 of Insulin like growth factor binding protein 3 (IGFBP3) gene were digested by HindIII and NlaIII restriction enzymes, respectively, in 90 mithun (Bos frontalis). The PCR-RFLP analysis showed the absence ofpolymorphism in these fragments in the animals screened. HindIII restriction enzyme produced two fragments of 269 and 127 bp in exon-5 of IGFl. Similarly maIII restriction enzyme produced two fragments of 551 and 101 bp in exon 2-3 of IGFBP3 gene. Two single nucleotide differences in IGFl while six such differences in IGFBP3 gene existed in mithun sequences when compared to cattle. The sequence of the amplicons, which were the first reports on these genes in mithun, were submitted to GenBank (Accession numbers EF686016 and EF686017, respectively).
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Date 2019-02-01T06:20:52Z
2019-02-01T06:20:52Z
2009
 
Type Research Paper
 
Identifier To cite this article: Manjit Panigrahi , Subodh Kumar , S. M. Deb , Abhijit Mitra , Arjava Sharma & K. M. Bujarbaruah (2009) Lack of Polymorphism in Partial Insulin Like Growth Factor 1 (IGF1) and Insulin Like Growth Factor Binding Protein 3 (IGFBP3) Genes of Mithun, Journal of Applied Animal Research, 36:1, 41-44, DOI: 10.1080/09712119.2009.9707027
0971-2119 (Print) 0974-1844 (Online)
http://krishi.icar.gov.in/jspui/handle/123456789/16676
 
Language English
 
Relation Not Available;
 
Publisher Taylor and Francis Group, LLC