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Abundance of antibiotic resistance genes in environmental bacteriophages.

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Title Abundance of antibiotic resistance genes in environmental bacteriophages.
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Creator Anand T, Bera BC, Vaid RK, Barua S, Riyesh T, Virmani N, Hussain M, Singh RK, Tripathi BN.
 
Subject Bacteriophage, antibiotic resistance genes, blaTEM, blaOXA-2, intI1, intI2, intI3, tetA and tetW
 
Description Not Available
The ecosystem is continuously exposed to a wide variety of antimicrobials through
waste effluents, agricultural run-offs and animal-related and anthropogenic
activities, which contribute to the spread of antibiotic resistance genes (ARGs).
The contamination of ecosystems with ARGs may create increased opportunities for
their transfer to naive microbes and eventually lead to entry into the human food
chain. Transduction is a significant mechanism of horizontal gene transfer in
natural environments, which has traditionally been underestimated as compared to
transformation. We explored the presence of ARGs in environmental bacteriophages
in order to recognize their contribution in the spread of ARGs in environmental
settings. Bacteriophages were isolated against environmental bacterial isolates,
purified and bulk cultured. They were characterized, and detection of ARG and
intI genes including blaTEM, blaOXA -2, intI1, intI2, intI3, tetA and tetW was
carried out by PCR. This study revealed the presence of various genes [tetA
(12.7 %), intI1 (10.9 %), intI2 (10.9 %), intI3 (9.1 %), tetW (9.1 %) and
blaOXA -2 (3.6 %)] and blaTEM in a significantly higher proportion (30.9 %).
blaSHV, blaOXA -1, tetO, tetB, tetG, tetM and tetS were not detected in any of the
phages. Soil phages were the most versatile in terms of ARG carriage. Also, the
relative abundance of tetA differed significantly vis-à-vis source. The phages
from organized farms showed varied ARGs as compared to the unorganized sector,
although blaTEM ARG incidences did not differ significantly. The study reflects
on the role of phages in dissemination of ARGs in environmental reservoirs, which
may provide an early warning system for future clinically relevant resistance
mechanisms.
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Date 2019-12-02T05:32:55Z
2019-12-02T05:32:55Z
2016-10-21
 
Type Journal
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/26182
 
Language English
 
Relation Not Available;
 
Publisher Not Available