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Genetic characterization of equine herpesvirus 1 isolates from abortion outbreaks in India.

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Title Genetic characterization of equine herpesvirus 1 isolates from abortion outbreaks in India.
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Creator Anagha G, Gulati BR, Riyesh T, Virmani N
 
Subject Equine herpesvirus1, Single nucleotide polymorphism, Neurophathogenic, Non-neurophathogenic
 
Description Not Available
Equine herpesvirus 1 (EHV1) is a common pathogen of horses that causes upper
respiratory tract disease, abortion, neonatal death and neurological disease. The
neurological form of disease is called equine herpesvirus myeloencephalopathy
(EHM). During the past decade, the incidence of EHM has been on the rise in
Europe, North America, Australia and Asia. Some EHV1 isolates causing EHM exhibit
a single-nucleotide polymorphism (SNP) in the DNA polymerase gene (ORF30) at
position 2254 (A2254 to G2254). Further, based on polymorphism in the ORF68, EHV1
isolates have been classified into different groups. The aim of the present study
was to estimate the genetic diversity of EHV1 and to determine the prevalence of
the neuropathogenic genotype of EHV1 in India. Out of 133 clinical specimens from
abortion cases in northern India, 56 were positive for EHV1 infection. Analysis
of the A/G SNP by real-time PCR and sequence analysis revealed that 54 of 56
samples (96.43 %) were of the non-neuropathogenic genotype (A2254), while two
(3.57 %) had the neuropathogenic marker (G2254). Sequence analysis of the
polymorphic region of ORF68 of EHV1 isolates (n = 9) from India indicated that
the Delhi/1998, Tohana-2/2013, Hisar-2/2014 and Hisar-15/1990 isolates belonged
to group 4, while the Jind/1996, Rajasthan/1998, Delhi-3/2007 and Tohana-5/1996
isolates clustered within group 5. One isolate (Hisar-7/1990) exhibited SNPs at
positions C710 and C713, forming a separate group. Here, we report for the first
time the detection of neuropathogenic genotypes of EHV1 in India and show that
Indian EHV1 isolates cluster within groups 4 and 5.
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Date 2018-11-29T10:23:35Z
2018-11-29T10:23:35Z
2016-10-03
 
Type Journal
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/14165
 
Language English
 
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Publisher Not Available