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Complexities in Isolation and Purification of Multiple Viruses from Mixed Viral Infections: Viral Interference, Persistence and Exclusion.

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Title Complexities in Isolation and Purification of Multiple Viruses from Mixed Viral Infections: Viral Interference, Persistence and Exclusion.
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Creator Kumar N, Barua S, Riyesh T, Chaubey KK, Rawat KD, Khandelwal N, Mishra AK, Sharma N, Chandel SS, Sharma S, Singh MK, Sharma DK, Singh SV, Tripathi BN.
 
Subject Peste des petits ruminants virus (PPRV), foot-and-mouth disease virus (FMDV), mixed infection,cytopathic effect (CPE)
 
Description Not Available
Successful purification of multiple viruses from mixed infections remains a
challenge. In this study, we investigated peste des petits ruminants virus (PPRV)
and foot-and-mouth disease virus (FMDV) mixed infection in goats. Rather than in
a single cell type, cytopathic effect (CPE) of the virus was observed in
cocultured Vero/BHK-21 cells at 6th blind passage (BP). PPRV, but not FMDV could
be purified from the virus mixture by plaque assay. Viral RNA (mixture)
transfection in BHK-21 cells produced FMDV but not PPRV virions, a strategy which
we have successfully employed for the first time to eliminate the
negative-stranded RNA virus from the virus mixture. FMDV phenotypes, such as
replication competent but noncytolytic, cytolytic but defective in plaque
formation and, cytolytic but defective in both plaque formation and standard FMDV
genome were observed respectively, at passage level BP8, BP15 and BP19 and hence
complicated virus isolation in the cell culture system. Mixed infection was not
found to induce any significant antigenic and genetic diversity in both PPRV and
FMDV. Further, we for the first time demonstrated the viral interference between
PPRV and FMDV. Prior transfection of PPRV RNA, but not Newcastle disease virus
(NDV) and rotavirus RNA resulted in reduced FMDV replication in BHK-21 cells
suggesting that the PPRV RNA-induced interference was specifically directed
against FMDV. On long-term coinfection of some acute pathogenic viruses (all
possible combinations of PPRV, FMDV, NDV and buffalopox virus) in Vero cells, in
most cases, one of the coinfecting viruses was excluded at passage level 5
suggesting that the long-term coinfection may modify viral persistence. To the
best of our knowledge, this is the first documented evidence describing a natural
mixed infection of FMDV and PPRV. The study not only provides simple and reliable
methodologies for isolation and purification of two epidemiologically and
economically important groups of viruses, but could also help in establishing
better guidelines for trading animals that could transmit further infections and
epidemics in disease free nations.
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Date 2018-11-29T10:22:55Z
2018-11-29T10:22:55Z
1001-01-01
 
Type Journal
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/14164
 
Language English
 
Relation Not Available;
 
Publisher Not Available