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Genetic characterization and phylogenetic analysis of host-range genes of Camelpox virus isolates from India

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Title Genetic characterization and phylogenetic analysis of host-range genes of Camelpox virus isolates from India
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Creator Bera BC, Barua S, Shanmugasundaram K, Anand T, Riyesh T, Vaid RK, Virmani N, Kundu S, Yadav NK, Malik P, Singh RK
 
Subject Camelpox virus, CMLV, Host-range gene, Orthopoxvirus, Concatenated phylogenetic tree
 
Description Not Available
Camelpox virus (CMLV), a close variant of variola virus (VARV) infects camels
worldwide. The zoonotic infections reported from India signify the need to study
the host-range genes-responsible for host tropism. We report sequence and
phylogenetic analysis of five host-range genes: cytokine response modifier B
(crmB), chemokine binding protein (ckbp), viral schlafen-like (v-slfn),
myxomavirus T4-like (M-T4-like) and b5r of CMLVs isolated from outbreaks in
India. Comparative analysis revealed that these genes are conserved among CMLVs
and shared 94.5-100 % identity at both nucleotide (nt) and amino acid (aa)
levels. All genes showed identity (59.3-98.4 %) with cowpox virus (CPXV) while
three genes-crmB, ckbp and b5r showed similarity (92-96.5 %) with VARVs at both
nt and aa levels. Interestingly, three consecutive serine residue insertions were
observed in CKBP protein of CMLV-Delhi09 isolate which was similar to CPXV-BR and
VACVs, besides five point mutations (K53Q, N67I, F84S, A127T and E182G) were also
similar to zoonotic OPXVs. Further, few inconsistent point mutation(s) were also
observed in other gene(s) among Indian CMLVs. These indicate that different
strains of CMLVs are circulating in India and these mutations could play an
important role in adaptation of CMLVs in humans. The phylogeny revealed
clustering of all CMLVs together except CMLV-Delhi09 which grouped separately due
to the presence of specific point mutations. However, the topology of the
concatenated phylogeny showed close evolutionary relationship of CMLV with VARV
and TATV followed by CPXV-RatGer09/1 from Germany. The availability of this
genetic information will be useful in unveiling new strategies to control
emerging zoonotic poxvirus infections.
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Date 2018-11-29T10:25:20Z
2018-11-29T10:25:20Z
2015-07-17
 
Type Journal
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/14168
 
Language English
 
Relation Not Available;
 
Publisher Not Available