Antioxidant and antimicrobial activity of camel milk casein hydrolysates and its fractions
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Title |
Antioxidant and antimicrobial activity of camel milk casein hydrolysates and its fractions
Not Available |
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Creator |
DEVENDRA KUMAR, MANISH KUMAR CHATLI, RAGHVENDAR SINGH, NITIN MEHTA and PAVAN KUMAR
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Subject |
Camel Milk
Enzymatic hydrolysis Ultra filtration Antioxidant activity Antimicrobial activity |
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Description |
Not Available
Camel milk casein hydrolysates by Alcalase, -Chymotrypsin and Papain were ultra-filtered and different fractions were assessed for antioxidant and antimicrobial activity. The casein hydrolysate fractions were analyzed for antioxidant activities viz. 2,2 -azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), 2,2 -diphenyl-1-picrylhydrazyl (DPPH) and Ferric reducing antioxidant power assay (FRAP), and antimicrobial activity by inhibition zone assay. In ABTS, DPPH and FRAP assay, the inhibition activity was recorded significantly (P < 0.05) higher for whole hydrolysares whereas among fractions F2 (1–5 kDa), F3 (5–10 kDa) of all the three hydrolysates had comperatively higher antioxidant activity. It was also observed that - Chymotrypsin could produce protein hydrolysates and fractions with higher antioxidant activities. The antimicrobial activity (zone of inhibition; mm) was also recorded higher for whole hydrolysates as compared to their fractions; however, among different fractions the inhibition zones were almost comparable. It was also observed that Alcalase and -Chymotrypsin could produce paptides with higher antimicrobial activity. The results suggested that camel milk casein hydrolysates could be fractioned to get specific molecular weight peptides, however, for food application or for direct human consumption, use of whole hydrolysates could be more beneficial with regards to its functionalities and cost of production. Not Available |
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Date |
2018-11-30T07:22:52Z
2018-11-30T07:22:52Z 2016-05-04 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/14464 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Elsevier
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