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Research on Freezability of Spermatozoa by Use of Various Concentrations of Glycerol in Mithun (Bos frontalis) Semen Cryopreservation

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Title Research on Freezability of Spermatozoa by Use of Various Concentrations of Glycerol in Mithun (Bos frontalis) Semen Cryopreservation
Not Available
 
Creator K. K. Baruah
M. Mondal
A. Dhali
B. Bora
A. Mech
A. Baruah
P. Perumal
K. C. Das
 
Subject Mithun semen, glycerol, cryopreservation
 
Description Not Available
A study was conducted to investigate the various glycerol concentrations on freezability
of mithun semen. The present study was conducted in semen production laboratory,
ICAR-NRC on Mithun, Medziphema, Nagaland, India. A total of 20 ejaculates were
collected from apparently healthy mithun bulls (n=6) by trans-rectal massage method.
These ejaculates were extended with tris–egg yolk-citrate-glycerol diluent (TECG) and
5, 6 and 7% (v/v) glycerol, respectively were used as a cryoprotectant. Extended semen
was equilibrated, cryopreserved and stored in liquid nitrogen with standard protocols.
Semen quality parameters (SQPs) such as percentage of forward progressive motility,
viability (Eosin Nigrosin staining), acrosomal integrity (Giemsa staining) and total
morphological abnormality (Eosin Nigrosin staining) were estimated with standard
procedure at different stage of preservation and with different concentration of the
glycerol. The result revealed that these semen quality parameters were differed nonsignificantly
among the three experimental groups at immediately after final dilution
(Stage: 1) and differed significantly (p
Not Available
 
Date 2019-02-01T06:22:11Z
2019-02-01T06:22:11Z
2016
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/16684
 
Language English
 
Relation Not Available;
 
Publisher International Journal of Bio-resource and Stress Management