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Use of nucleic acid recognition methods (m-PCR and RT-LAMP) for the detection of foot-and-mouth disease virus excreted in cow milk

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Title Use of nucleic acid recognition methods (m-PCR and RT-LAMP) for the detection of foot-and-mouth disease virus excreted in cow milk
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Creator RAJEEV RANJAN1, JITENDRA KUMAR BISWAL2, GAURAV KUMAR SHARMA3, AJAY KUMAR SHARMA4, KARAM PAL SINGH5, JYOTI MISRI6 and BRAMHADEV PATTNAIK7
 
Subject Cow milk, FMD virus, Nucleic acid recognition method, Virus isolation
 
Description Not Available
The present study was undertaken to see the excretion of FMD virus in milk during and after the subset of FMD
outbreak. Fore-milk (50 ml) was sampled from 12 clinically infected and 3 asymptomatic cows in the morning.
Analytical sensitivity of NAR methods was estimated using uninfected negative milk sample spiked with 105.7
TCID50/ml FMD serotype O virus (IND R2/1975) in 10 fold serial dilution. Detection limit of mPCR and TLAMP
assay was 102.7 and 101.7 TCID50/ml, respectively. 15 individual and pooled cows’ milk samples infected
with FMD virus were processed for virus isolation (VI) and detection till 37 days post clinical manifestation (dpm). Virus isolation from individual and pooled milk from infected cow was positive till 6 and 4 dpm, respectively. Individual milk and pooled milk samples were found positive by m-PCR till 37 and 14 dpm, respectively, but by RT-LAMP till 37 and 21 dpm, respectively. In case of asymptomatic cows, viral genome was detected 2–5 days before appearance of disease in other animals. Milk virus isolate had 100% nucleotide identity at VP1 coding region. mPCR and RT LAMP assays has potential to detect FMD virus in milk and help to prevent the spread of FMD virus from one place to another place.
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Date 2018-12-04T00:53:57Z
2018-12-04T00:53:57Z
2016-08-01
 
Type Research Paper
 
Identifier Ranjan R, Biswal JK, Sharma GK, Sharma AK, Singh KP, Misri J and Pattnaik B. (2016). Use of nucleic acid recognition methods (m-PCR and RT-LAMP) for the detection of foot-and-mouth disease virus excreted in cow milk. Indian J Anim Sci. 86 (8): 865–868.
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http://krishi.icar.gov.in/jspui/handle/123456789/15520
 
Language English
 
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Publisher Not Available