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Microarray analysis of gene expression in parthenotes and in vitro–derived goat embryos

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Title Microarray analysis of gene expression in parthenotes and in vitro–derived goat embryos
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Creator Singh R, Kumar K, Mahapatra PS, Kumar M, Agarwal P, Bhure SK, Malakar D, Bhanja SK, Bag S.
 
Subject Goat embryo
Gene expression
IVF
Microarray analysis
Parthenogenesis
 
Description Not Available
The present work was carried out to investigate the global gene expression profile to
search deferentially expressed candidate transcripts between parthenogenetic and
in vitro–fertilized (IVF) caprine morula. For this study, total RNA was isolated from diploid
parthenogenetic and IVF embryos, and complementary DNA was synthesized. Microarray
and relative real-time polymerase chain reaction analysis were performed to check global
gene expression profile and validation, respectively. According to the microarray analysis,
the total number of up regulated (UR) and down regulated (DR) genes was 613 and 220,
respectively in diploid parthenogenetic morula as compared with IVF morula. The number
of genes showing about two-, two- to five-, five- to 10-, 10- to 20-, and above 20-fold UR
and DR genes was 147, 229, 122, 59, and 56 and 94, 73, 18, 13, and 22, respectively. Five UR
genes validated (PTEN, PHF3, CTNNB1, SELK, and NPDC1) and all of them were significantly
higher in parthenotes, which was in accordance with microarray results, whereas the
expression of DR (AURKC and KLF15) genes were down regulated in parthenotes as
observed in microarray results but the difference was not significant (P < 0.05). In
conclusion, our findings demonstrate differential expression of a large number of genes in
parthenotes compared with IVF embryos, which may be the reason for aberrant parthenogenetic
embryo development in caprine species.
National Agricultural Innovative Project (NAIP), Indian Council of Agricultural Research (ICAR), Government of India.
 
Date 2019-04-08T06:07:13Z
2019-04-08T06:07:13Z
2014-01-11
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/18060
 
Language English
 
Relation Not Available;
 
Publisher Elsevier