Evaluation of a novel outer membrane surface-exposed protein, LIC13341 of Leptospira, as an adhesin and serodiagnostic candidate marker for leptospirosis.
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Title |
Evaluation of a novel outer membrane surface-exposed protein, LIC13341 of Leptospira, as an adhesin and serodiagnostic candidate marker for leptospirosis.
Not Available |
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Creator |
Ghosh KK
Prakash A Shrivastav P Balamurugan V Kumar M. |
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Subject |
ELISA
Leptospira antibodies extracellular matrix component lipoprotein recombinant protein |
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Description |
Not Available
The outer membrane proteins of the pathogen are targeted to understand host-pathogen interactions and are central to the development of diagnostics. We report that Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 contains a gene LIC13341 that encodes a conserved outer membrane/periplasmic lipoprotein. The gene LIC13341 was cloned into expression vector pET28a and the recombinant LIC13341 (r-LIC13341) protein was purified from Escherichia coli BL21 (DE3) using affinity chromatography. The secondary structure of the purified r-LIC13341 protein featured a typical β-strand when observed by circular dichroism spectroscopy. Immunoblotting using antibodies raised against r-LIC13341 in BALB/c mice can detect LIC13341 expression in the Leptospira lysates and suggested that antigen LIC13341 is immunogenic. Phase separation and protease assays determined that LIC13341 is a surface-exposed outer membrane protein of Leptospira. The r-LIC13341 can bind to a wide spectrum of host extracellular matrices (ECMs). The specific adherence of Leptospira to laminin and hyaluronic acid of the ECM was competitively inhibited in the presence of r-LIC13341. The enzyme-linked immunosorbent assay and immunoblot performed using human or bovine leptospirosis serum (n=50) recognized r-LIC13341, suggesting that LIC13341 is expressed in diverse hosts during Leptospira infection. Thus, the present finding suggests that the Leptospira LIC13341 antigen is a versatile outer membrane adhesin of diagnostic importance. Not Available |
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Date |
2019-05-25T03:15:12Z
2019-05-25T03:15:12Z 2018-07-03 |
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Type |
Research Paper
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Identifier |
Not Available
2996-9088 http://krishi.icar.gov.in/jspui/handle/123456789/19842 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Microbiology society
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