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Molecular epidemiological analysis of wild animal rabies isolates from India

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Title Molecular epidemiological analysis of wild animal rabies isolates from India
Not Available
 
Creator Manjunatha Reddy GB
Singh R
Singh KP
Sharma AK
Vineetha S
Saminathan M
Sajjanar B
 
Subject India
nucleoprotein gene
phylogenetic analysis
rabies virus
wild animals
 
Description Not Available
Aim: This study was conducted to know the genetic variability of rabies viruses (RVs) from wild animals in India.

Materials and Methods: A total of 20 rabies suspected brain samples of wild animals from different states of India were included in the study. The samples were subjected for direct fluorescent antibody test (dFAT), reverse transcription polymerase chain reaction (RT-PCR), and quantitative reverse transcriptase real-time PCR (RT-qPCR). The phylogenetic analysis of partial nucleoprotein gene sequences was performed.

Results: Of 20 samples, 11, 10, and 12 cases were found positive by dFAT, RT-PCR, and RT-qPCR, respectively. Phylogenetic analysis showed that all Indian wild RVs isolates belonged to classical genotype 1 of Lyssavirus and were closely related to Arctic/Arctic-like single cluster indicating the possibility of a spillover of rabies among different species.

Conclusion: The results indicated the circulation of similar RVs in sylvatic and urban cycles in India. However, understanding the role of wild animals as reservoir host needs to be studied in India.
Not Available
 
Date 2019-05-25T03:50:57Z
2019-05-25T03:50:57Z
2019-03-04
 
Type Research Paper
 
Identifier Not Available
2231-0916
http://krishi.icar.gov.in/jspui/handle/123456789/19851
 
Language English
 
Relation Not Available;
 
Publisher Veterinary World