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Phenotypic and molecular characterization of extended spectrum β-lactamase, ampc β-lactamase and metallo β-lactamase producing Klebsiella spp. from farm animals in India

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Title Phenotypic and molecular characterization of extended spectrum β-lactamase, ampc β-lactamase and metallo β-lactamase producing Klebsiella spp. from farm animals in India
Not Available
 
Creator Tewari R
Mitra SD
Venugopal N
Das S
Ganaie F
Sen A
Shome R
Rahman H
Shome BR
 
Subject Antibiotic resistance
Klebsiella spp.
Livestock
Mobile genetic elements
 
Description Not Available
Animal populace has attained less attention in antimicrobial resistance research than human sector resulting in limited information available on animal origin isolates. The study aimed to investigate the occurrence of ESBL, AmpC and MBL genes, plasmids and integrons in Klebsiella spp. Fecal samples were collected from healthy livestock (cattle, pig, sheep and goat) and poultry between 2012-2015. Preliminary identification of isolates was done by conventional phenotypic methods and confirmed by genotypic methods. Antimicrobial susceptibility testing was performed by disk diffusion method. Molecular characterization by PCR was conducted for 17 antimicrobial resistance genes, 3 integrons and 18 Plasmid replicons. A total of 48 Klebsiella isolates were identified. Multidrug resistance was observed in 23% of isolates. ESBL, AmpC and MBL resistance genes were detected in 21%, 6% and 4% of isolates, respectively. Integrons [Int2 gene] and plasmids [Y and IncI genes] were identified in 6% and 8% of isolates.The study highlights the existence of ESBL, AmpC and MBL producing Klebsiella isolates with certain strains carrying mobile genetic elements in healthy livestock and poultry as reservoirs and probable disseminators of resistance, thus imposing public health threat. Prudent use of antimicrobials and continuous intensified surveillance in animal sector is crucial to limit the spread of such emerging resistant traits.
Not Available
 
Date 2019-05-27T06:19:10Z
2019-05-27T06:19:10Z
2018-09-18
 
Type Research Paper
 
Identifier Not Available
0976-0555
http://krishi.icar.gov.in/jspui/handle/123456789/19899
 
Language English
 
Relation Not Available;
 
Publisher Indian Journal of Animal Research