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Dissemination and characterization of extended spectrum β-Lactamase, AmpC β-Lactamase and Metallo-β Lactamase producing Escherichia coli from livestock and poultry in North East India: A molecular surveillance approach

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Title Dissemination and characterization of extended spectrum β-Lactamase, AmpC β-Lactamase and Metallo-β Lactamase producing Escherichia coli from livestock and poultry in North East India: A molecular surveillance approach
Not Available
 
Creator Tewari R
Mitra SD
Ganaie F
Das S
Chakraborty A
Venugopal N
Shome R
Rahman H
Shome BR
 
Subject AmpC β lactamase
Antimicrobial resistance
Escherichia coli
Extended-spectrum β-lactamase
India
Metallo-β-lactamase
 
Description Not Available
OBJECTIVES:
The aim of this study was to identify and characterise probable extended-spectrum β-lactamase (ESBL)-, AmpC lactamase- and/or metallo-β-lactamase (MBL)-producing Escherichia coli variants circulating in the livestock and poultry environment to establish their epidemiological significance, genetic diversity, antimicrobial resistance (AMR) trends and virulence.
METHODS:
The culture method and E. coli-specific multiplex PCR identified 78 E. coli strains from faecal samples of healthy livestock and poultry. The antibiogram was determined by the disk diffusion and minimum inhibitory concentration (MIC) methods. Antimicrobial-resistant E. coli isolates were screened for the presence of ESBL, AmpC and MBL genes. Isolates were further characterised by plasmid replicon typing, integron assay and virulence gene analysis. Genetic diversity was assessed by random amplification of polymorphic DNA (RAPD) analysis and multilocus sequence typing (MLST).
RESULTS:
ESBL (CTX-M group 1, CTX-M group 4, TEM), AmpC (EBC, FOX, CMY, DHA) and MBL (IMP, SIM) resistance determinants were identified in 75%, 19% and 6% of isolates, respectively. Nine plasmid replicon types were distributed among resistant E. coli strains, with the most common plasmid replicon types being L/M and Y. Integrons were detected in 19% of E. coli isolates. RAPD analysis categorised the E. coli isolates into three clusters. MLST revealed seven different sequence types (STs), with ST10 being the most common.
CONCLUSIONS:
This study demonstrated a high prevalence of animals carrying potential ESBL- and AmpC-producing E. coli and emphasises the need for rigorous surveillance in the animal sector to identify critical control points conducive to prevent the rapid dissemination of AMR.
Not Available
 
Date 2019-05-27T06:27:19Z
2019-05-27T06:27:19Z
2019-01-08
 
Type Research Paper
 
Identifier Not Available
2213-7173
http://krishi.icar.gov.in/jspui/handle/123456789/19900
 
Language English
 
Relation Not Available;
 
Publisher Elsevier Ltd.